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Structural basis of nanobody recognition of grapevine fanleaf virus and of virus resistance loss

Igor Orlov 1 Caroline Hemmer 2, 3 Léa Ackerer 2, 3 Bernard Lorber Ahmed Ghannam 2 Vianney Poignavent 2 Kamal Hleibieh 2 Claude Sauter 4 Corinne Schmitt-Keichinger 2, 3 Lorène Belval 3 Jean-Michel Hily 3, 5 Aurélie Marmonier 3 Véronique Komar 3 Sophie Gersch 3 Pascale Schellenberger 2, 3 Patrick Bron 6 Emmanuelle Vigne 3 Serge Muyldermans 7 Olivier Lemaire 3 Gérard Demangeat 3 Christophe Ritzenthaler 2 Bruno Klaholz 1
1 Inserm U964 - CNRS UMR7104 - IGBMC - Centre for Integrative Biology - CBI
IGBMC - Institut de Génétique et de Biologie Moléculaire et Cellulaire
2 IBMP - Institut de biologie moléculaire des plantes
3 SVQV - Santé de la vigne et qualité du vin
4 ARN - Architecture et Réactivité de l'ARN
5 Institut Français de la Vigne et du Vin [Le Grau du Roi]
6 CBS - Centre de Biochimie Structurale [Montpellier]
7 VUB - Vrije Universiteit Brussel
Abstract : Grapevine fanleaf virus (GFLV) is a picorna-like plant virus transmitted by nematodes that affects vineyards worldwide. Nanobody (Nb)-mediated resistance against GFLV has been created recently, and shown to be highly effective in plants, including grapevine, but the underlying mechanism is unknown. Here we present the high-resolution cryo electron microscopy structure of the GFLV-Nb23 complex, which provides the basis for molecular recognition by the Nb. The structure reveals a composite binding site bridging over three domains of one capsid protein (CP) monomer. The structure provides a precise mapping of the Nb23 epitope on the GFLV capsid in which the antigen loop is accommodated through an induced-fit mechanism. Moreover, we uncover and characterize several resistance-breaking GFLV isolates with amino acids mapping within this epitope, including C-terminal extensions of the CP, which would sterically interfere with Nb binding. Escape variants with such extended CP fail to be transmitted by nematodes linking Nb-mediated resistance to vector transmission. Together, these data provide insights into the molecular mechanism of Nb23-mediated recognition of GFLV and of virus resistance loss.
Keywords : GFLV nanobody structural biology virus.
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https://www.hal.inserm.fr/inserm-03103234
Contributor : Bruno Klaholz Connect in order to contact the contributor
Submitted on : Friday, January 8, 2021 - 7:33:00 AM
Last modification on : Wednesday, November 3, 2021 - 8:00:59 AM
Long-term archiving on: : Friday, April 9, 2021 - 6:16:01 PM

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INSERM | IGBMC | UNIV-MONTPELLIER | CNRS | INRAE | BS | SITE-ALSACE

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Igor Orlov, Caroline Hemmer, Léa Ackerer, Bernard Lorber, Ahmed Ghannam, et al.. Structural basis of nanobody recognition of grapevine fanleaf virus and of virus resistance loss. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2020, 117 (20), pp.10848-10855. ⟨10.1073/pnas.1913681117⟩. ⟨inserm-03103234⟩

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