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Efficient Therapeutic Delivery by a Novel Cell-Penetrating Peptide Derived from Acinus

Abstract : In this study, we have identified a novel cell-penetrating sequence, termed hAP10, from the C-terminus of the human protein Acinus. hAP10 was able to efficiently enter various normal and cancerous cells, likely through an endocytosis pathway, and to deliver an EGFP cargo to the cell interior. Cell penetration of a peptide, hAP10DR, derived from hAP10 by mutation of an aspartic acid residue to an arginine was dramatically increased. Interestingly, a peptide containing a portion of the heptad leucine repeat region domain of the survival protein AAC-11 (residues 377-399) fused to either hAP10 or hAP10DR was able to induce tumor cells, but not normal cells, death both ex vivo on Sézary patients' circulating cells and to inhibit tumor growth in vivo in a sub-cutaneous xenograft mouse model for the Sézary syndrome. Combined, our results indicate that hAP10 and hAP10DR may represent promising vehicles for the in vitro or in vivo delivery of bioactive cargos, with potential use in clinical settings.
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https://www.hal.inserm.fr/inserm-03024400
Contributor : Anne Marie-Cardine <>
Submitted on : Wednesday, November 25, 2020 - 6:17:24 PM
Last modification on : Wednesday, June 2, 2021 - 4:27:14 PM
Long-term archiving on: : Friday, February 26, 2021 - 8:00:17 PM

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Justine Habault, Claire Fraser, Ewa Pasquereau-Kotula, Maëlys Born-Bony, Anne Marie-Cardine, et al.. Efficient Therapeutic Delivery by a Novel Cell-Penetrating Peptide Derived from Acinus. Cancers, MDPI, 2020, 12 (7), pp.1858. ⟨10.3390/cancers12071858⟩. ⟨inserm-03024400⟩

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