CDKN2A/p16INK4a suppresses hepatic fatty acid oxidation through the AMPKα2-SIRT1-PPARα signaling pathway - Archive ouverte HAL Access content directly
Journal Articles Journal of Biological Chemistry Year : 2021

CDKN2A/p16INK4a suppresses hepatic fatty acid oxidation through the AMPKα2-SIRT1-PPARα signaling pathway

(1) , (1) , (1) , (1) , (1) , (1) , (1) , (1) , (1) , (1) , (2) , (1) , (3) , (1) , (2) , (4) , (4) , (1) , (2) , (1) , (1) , (1)
1
2
3
4

Abstract

In addition to their well-known role in the control of cellular proliferation and cancer, cell cycle regulators are increasingly identified as important metabolic modulators. Several GWAS have identified SNPs near CDKN2A, the locus encoding for p16INK4a (p16), associated with elevated risk for cardiovascular diseases and type-2 diabetes development, two pathologies associated with impaired hepatic lipid metabolism. Although p16 was recently shown to control hepatic glucose homeostasis, it is unknown whether p16 also controls hepatic lipid metabolism. Using a combination of in vivo and in vitro approaches, we found that p16 modulates fasting-induced hepatic fatty acid oxidation (FAO) and lipid droplet accumulation. In primary hepatocytes, p16-deficiency was associated with elevated expression of genes involved in fatty acid catabolism. These transcriptional changes led to increased FAO and were associated with enhanced activation of PPARα through a mechanism requiring the catalytic AMPKα2 subunit and SIRT1, two known activators of PPARα. By contrast, p16 overexpression was associated with triglyceride accumulation and increased lipid droplet numbers in vitro, and decreased ketogenesis and hepatic mitochondrial activity in vivo. Finally, gene expression analysis of liver samples from obese patients revealed a negative correlation between CDKN2A expression and PPARA and its target genes. Our findings demonstrate that p16 represses hepatic lipid catabolism during fasting and may thus participate in the preservation of metabolic flexibility.
Fichier principal
Vignette du fichier
1-s2.0-S0021925817506183-main.pdf (3.06 Mo) Télécharger le fichier
Origin : Publication funded by an institution

Dates and versions

inserm-03019831 , version 1 (23-11-2020)
inserm-03019831 , version 2 (01-07-2021)

Licence

Attribution - CC BY 4.0

Identifiers

Cite

Yann Deleye, Alexia Karen Cotte, Sarah Anissa Hannou, Nathalie Hennuyer, Lucie Bernard, et al.. CDKN2A/p16INK4a suppresses hepatic fatty acid oxidation through the AMPKα2-SIRT1-PPARα signaling pathway. Journal of Biological Chemistry, 2021, 295 (50), pp.17310-17322. ⟨10.1074/jbc.RA120.012543⟩. ⟨inserm-03019831v2⟩
84 View
98 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More