Skip to Main content Skip to Navigation
Journal articles

Mitochondria transfer from tumor-activated stromal cells (TASC) to primary Glioblastoma cells

Céline Salaud 1 Arturo Alvarez-Arenas 2 Fanny Geraldo 1 Juan Belmonte-Beitia 2 Gabriel Calvo 2 Catherine Gratas 3 Claire Pecqueur 3 Delphine Garnier 1 Victor Pérez-Garcià 2 François Vallette 1 Lisa Oliver 1, *
* Corresponding author
1 CRCINA-ÉQUIPE 9 - Apoptosis and Tumor Progression
CRCINA - Centre de Recherche en Cancérologie et Immunologie Nantes-Angers
3 CRCINA-ÉQUIPE 14 - Endothelium Radiobiology and Targeting
CRCINA - Centre de Recherche en Cancérologie et Immunologie Nantes-Angers
Abstract : The tumor microenvironment (TME) controls many aspects of cancer development but little is known about its effect in Glioblastoma (GBM), the main brain tumor in adults. Tumor-activated stromal cell (TASC) population, a component of TME in GBM, was induced in vitro by incubation of MSCs with culture media conditioned by primary cultures of GBM under 3D/organoid conditions. We observed mito-chondrial transfer by Tunneling Nanotubes (TNT), extracellular vesicles (EV) and cannibalism from the TASC to GBM and analyzed its effect on both proliferation and survival. We created primary cultures of GBM or TASC in which we have eliminated mitochondrial DNA [Rho 0 (r 0) cells]. We found that TASC, as described in other cancers, increased GBM proliferation and resistance to standard treatments (radio-therapy and chemotherapy). We analyzed the incorporation of purified mitochondria by r 0 and r þ cells and a derived mathematical model taught us that r þ cells incorporate more rapidly pure mitochondria than r 0 cells.
Document type :
Journal articles
Complete list of metadatas

Cited literature [33 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-03013565
Contributor : Elizabeth Bernardo <>
Submitted on : Thursday, November 19, 2020 - 8:39:07 AM
Last modification on : Friday, November 20, 2020 - 3:23:14 AM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

Collections

Citation

Céline Salaud, Arturo Alvarez-Arenas, Fanny Geraldo, Juan Belmonte-Beitia, Gabriel Calvo, et al.. Mitochondria transfer from tumor-activated stromal cells (TASC) to primary Glioblastoma cells. Biochemical and Biophysical Research Communications, Elsevier, 2020, 533, pp.139 - 147. ⟨10.1016/j.bbrc.2020.08.101⟩. ⟨inserm-03013565⟩

Share

Metrics

Record views

41