Skip to Main content Skip to Navigation
Journal articles

Glutamine uptake and utilization of human mesenchymal glioblastoma in orthotopic mouse model

Abstract : Background: Glioblastoma (GBM) are highly heterogeneous on the cellular and molecular basis. It has been proposed that glutamine metabolism of primary cells established from human tumors discriminates aggressive mesenchymal GBM subtype to other subtypes. Methods: To study glutamine metabolism in vivo, we used a human orthotopic mouse model for GBM. Tumors evolving from the implanted primary GBM cells expressing different molecular signatures were analyzed using mass spectrometry for their metabolite pools and enrichment in carbon 13 (13C) after 13C-glutamine infusion. Results: Our results showed that mesenchymal GBM tumors displayed increased glutamine uptake and utilization compared to both control brain tissue and other GBM subtypes. Furthermore, both glutamine synthetase and transglutaminase-2 were expressed accordingly to GBM metabolic phenotypes. Conclusion: Thus, our results outline the specific enhanced glutamine flux in vivo of the aggressive mesenchymal GBM subtype.
Document type :
Journal articles
Complete list of metadata

Cited literature [32 references]  Display  Hide  Download
Contributor : Elizabeth Bernardo Connect in order to contact the contributor
Submitted on : Thursday, November 19, 2020 - 8:33:33 AM
Last modification on : Wednesday, September 7, 2022 - 11:32:04 AM


Publisher files allowed on an open archive



Kristell Oizel, Chendong Yang, Ophelie Renoult, Fabien Gautier, Quyen N Do, et al.. Glutamine uptake and utilization of human mesenchymal glioblastoma in orthotopic mouse model. Cancer and Metabolism, 2020, 8, pp.9. ⟨10.1186/s40170-020-00215-8⟩. ⟨inserm-03013555⟩



Record views


Files downloads