Skip to Main content Skip to Navigation
Journal articles

Glutamine uptake and utilization of human mesenchymal glioblastoma in orthotopic mouse model

Abstract : Background: Glioblastoma (GBM) are highly heterogeneous on the cellular and molecular basis. It has been proposed that glutamine metabolism of primary cells established from human tumors discriminates aggressive mesenchymal GBM subtype to other subtypes. Methods: To study glutamine metabolism in vivo, we used a human orthotopic mouse model for GBM. Tumors evolving from the implanted primary GBM cells expressing different molecular signatures were analyzed using mass spectrometry for their metabolite pools and enrichment in carbon 13 (13C) after 13C-glutamine infusion. Results: Our results showed that mesenchymal GBM tumors displayed increased glutamine uptake and utilization compared to both control brain tissue and other GBM subtypes. Furthermore, both glutamine synthetase and transglutaminase-2 were expressed accordingly to GBM metabolic phenotypes. Conclusion: Thus, our results outline the specific enhanced glutamine flux in vivo of the aggressive mesenchymal GBM subtype.
Document type :
Journal articles
Complete list of metadatas

Cited literature [32 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-03013555
Contributor : Elizabeth Bernardo <>
Submitted on : Thursday, November 19, 2020 - 8:33:33 AM
Last modification on : Friday, November 20, 2020 - 3:23:13 AM

File

Oizel_2020Eq9+14.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Kristell Oizel, Chendong Yang, Ophelie Renoult, Fabien Gautier, Quyen Do, et al.. Glutamine uptake and utilization of human mesenchymal glioblastoma in orthotopic mouse model. Cancer and Metabolism, 2020, 8, pp.9. ⟨10.1186/s40170-020-00215-8⟩. ⟨inserm-03013555⟩

Share

Metrics

Record views

20

Files downloads

76