Glutamine uptake and utilization of human mesenchymal glioblastoma in orthotopic mouse model - Archive ouverte HAL Access content directly
Journal Articles Cancer and Metabolism Year : 2020

Glutamine uptake and utilization of human mesenchymal glioblastoma in orthotopic mouse model

(1) , (1) , (2) , (3, 4) , (1) , (5, 6) , (1) , (1) , (5, 4) , (1) , (2, 4) , (7, 1) , (2, 6)
1
2
3
4
5
6
7

Abstract

Background: Glioblastoma (GBM) are highly heterogeneous on the cellular and molecular basis. It has been proposed that glutamine metabolism of primary cells established from human tumors discriminates aggressive mesenchymal GBM subtype to other subtypes. Methods: To study glutamine metabolism in vivo, we used a human orthotopic mouse model for GBM. Tumors evolving from the implanted primary GBM cells expressing different molecular signatures were analyzed using mass spectrometry for their metabolite pools and enrichment in carbon 13 (13C) after 13C-glutamine infusion. Results: Our results showed that mesenchymal GBM tumors displayed increased glutamine uptake and utilization compared to both control brain tissue and other GBM subtypes. Furthermore, both glutamine synthetase and transglutaminase-2 were expressed accordingly to GBM metabolic phenotypes. Conclusion: Thus, our results outline the specific enhanced glutamine flux in vivo of the aggressive mesenchymal GBM subtype.
Fichier principal
Vignette du fichier
Oizel_2020Eq9+14.pdf (1.86 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Loading...

Dates and versions

inserm-03013555 , version 1 (19-11-2020)

Identifiers

Cite

Kristell Oizel, Chendong Yang, Ophelie Renoult, Fabien Gautier, Quyen N Do, et al.. Glutamine uptake and utilization of human mesenchymal glioblastoma in orthotopic mouse model. Cancer and Metabolism, 2020, 8, pp.9. ⟨10.1186/s40170-020-00215-8⟩. ⟨inserm-03013555⟩
67 View
44 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More