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Journal articles
A single early-in-life antibiotic course increases susceptibility to DSS-induced colitis
Abstract : Background: There is increasing evidence that the intestinal microbiota plays a crucial role in the maturation of the immune system and the prevention of diseases during childhood. Early-life short-course antibiotic use may affect the progression of subsequent disease conditions by changing both host microbiota and immunologic development. Epidemiologic studies provide evidence that early-life antibiotic exposures predispose to inflammatory bowel disease (IBD).
Methods: By using a murine model of dextran sodium sulfate (DSS)-induced colitis, we evaluated the effect on disease outcomes of early-life pulsed antibiotic treatment (PAT) using tylosin, a macrolide and amoxicillin, a beta-lactam. We evaluated microbiota effects at the 16S rRNA gene level, and intestinal T cells by flow cytometry. Antibiotic-perturbed or control microbiota were transferred to pups that then were challenged with DSS.
Results: A single PAT course early-in-life exacerbated later DSS-induced colitis by both perturbing the microbial community and altering mucosal immune cell composition. By conventionalizing germ-free mice with either antibiotic-perturbed or control microbiota obtained 40 days after the challenge ended, we showed the transferrable and direct effect of the still-perturbed microbiota on colitis severity in the DSS model.
Conclusions: The findings in this experimental model provide evidence that early-life microbiota perturbation may increase risk of colitis later in life.
Cited literature [77 references]
https://www.hal.inserm.fr/inserm-03010073
Contributor : Claire Roubaud-Baudron <>
Submitted on : Tuesday, November 17, 2020 - 3:29:46 PM
Last modification on : Wednesday, February 3, 2021 - 9:22:02 AM
Contributor : Claire Roubaud-Baudron <>
Submitted on : Tuesday, November 17, 2020 - 3:29:46 PM
Last modification on : Wednesday, February 3, 2021 - 9:22:02 AM
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s13073-020-00764-z.pdf
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