Primary Disseminated Multifocal Ewing Sarcoma: Results of the Euro-EWING 99 Trial, Journal of Clinical Oncology, vol.28, issue.20, pp.3284-3291, 2010. ,
International osteosarcoma incidence patterns in children and adolescents, middle ages and elderly persons, International Journal of Cancer, vol.125, issue.1, pp.229-234, 2009. ,
Osteosarcoma incidence and survival rates from 1973 to 2004, Cancer, vol.115, issue.7, pp.1531-1543, 2009. ,
Prognostic factors for local and distant control in Ewing sarcoma family of tumors, Annals of Oncology, vol.19, issue.4, pp.814-820, 2008. ,
Current questions in bone sarcomas, Current Opinion in Oncology, vol.30, p.1, 2018. ,
Gene fusion with an ETS DNA-binding domain caused by chromosome translocation in human tumours, Nature, vol.359, issue.6391, pp.162-165, 1992. ,
The Ewing Family of Tumors -- A Subgroup of Small-Round-Cell Tumors Defined by Specific Chimeric Transcripts, New England Journal of Medicine, vol.331, issue.5, pp.294-299, 1994. ,
EWS-FLI1 and EWS-ERG Gene Fusions Are Associated With Similar Clinical Phenotypes in Ewing's Sarcoma, Journal of Clinical Oncology, vol.17, issue.6, pp.1809-1809, 1999. ,
The extracellular matrix: A dynamic niche in cancer progression, Journal of Cell Biology, vol.196, issue.4, pp.395-406, 2012. ,
Accessories to the Crime: Functions of Cells Recruited to the Tumor Microenvironment, Cancer Cell, vol.21, issue.3, pp.309-322, 2012. ,
Bone microenvironment has an influence on the histological response of osteosarcoma to chemotherapy: Retrospective analysis and preclinical modeling, Am. J. Cancer Res, vol.7, pp.2333-2349, 2017. ,
URL : https://hal.archives-ouvertes.fr/inserm-01680800
Bone microenvironment signals in osteosarcoma development, Cellular and Molecular Life Sciences, vol.72, issue.16, pp.3097-3113, 2015. ,
Microenvironmental regulation of therapeutic response in cancer, Trends in Cell Biology, vol.25, issue.4, pp.198-213, 2015. ,
Microenvironmental regulation of tumor progression and metastasis, Nature Medicine, vol.19, issue.11, pp.1423-1437, 2013. ,
The Impact of Microenvironment on the Synovial Sarcoma Transcriptome, Cancer Microenvironment, vol.10, issue.1-3, pp.1-7, 2017. ,
An orthotopic xenograft model with survival hindlimb amputation allows investigation of the effect of tumor microenvironment on sarcoma metastasis, Clinical & Experimental Metastasis, vol.32, issue.7, pp.703-715, 2015. ,
Acidosis Promotes Metastasis Formation by Enhancing Tumor Cell Motility, Advances in Experimental Medicine and Biology, vol.876, pp.215-220, 2016. ,
Differential expression of mitotic regulators and tumor microenvironment influences the regional growth pattern of solid sarcoma along the cranio-caudal axis, Experimental Cell Research, vol.340, issue.1, pp.91-101, 2016. ,
Therapeutic Relevance of Osteoprotegerin Gene Therapy in Osteosarcoma: Blockade of the Vicious Cycle between Tumor Cell Proliferation and Bone Resorption, Cancer Research, vol.67, issue.15, pp.7308-7318, 2007. ,
Osteoprotegerin inhibits bone resorption and prevents tumor development in a xenogenic model of Ewing's sarcoma by inhibiting RANKL, Journal of Bone Oncology, vol.2, issue.3, pp.95-104, 2013. ,
Ewing sarcoma cells express RANKL and support osteoclastogenesis, The Journal of Pathology, vol.225, issue.2, pp.195-202, 2011. ,
Zoledronic acid inhibits osteosarcoma growth in an orthotopic model, Molecular Cancer Therapeutics, vol.6, issue.12, pp.3263-3270, 2007. ,
Enhanced tumor regression and tissue repair when zoledronic acid is combined with ifosfamide in rat osteosarcoma, Bone, vol.37, issue.1, pp.74-86, 2005. ,
Zoledronic Acid as a New Adjuvant Therapeutic Strategy for Ewing's Sarcoma Patients, Cancer Research, vol.70, issue.19, pp.7610-7619, 2010. ,
Zoledronic acid suppresses lung metastases and prolongs overall survival of osteosarcoma-bearing mice, Cancer, vol.104, issue.11, pp.2522-2529, 2005. ,
CXCR7 maintains osteosarcoma invasion after CXCR4 suppression in bone marrow microenvironment, Tumor Biology, vol.39, issue.5, p.101042831770163, 2017. ,
Clinicopathological and prognostic significance of chemokine receptor CXCR4 in patients with bone and soft tissue sarcoma: a meta-analysis, Clinical and Experimental Medicine, vol.17, issue.1, pp.59-69, 2015. ,
Tumor progression in osteosarcoma (OS): Role of the chemokine receptor CXCR4 and of its ligand stromal-cell derived factor 1 (SDF-1), Journal of Clinical Oncology, vol.22, issue.14_suppl, pp.9021-9021, 2004. ,
Tumor-Activated Mesenchymal Stromal Cells Promote Osteosarcoma Stemness and Migratory Potential via IL-6 Secretion, PLOS ONE, vol.11, issue.11, p.e0166500, 2016. ,
Interleukin-6 suppression reduces tumour self-seeding by circulating tumour cells in a human osteosarcoma nude mouse model, Oncotarget, vol.7, issue.1, pp.446-458, 2015. ,
Exosomes Derived from Human Bone Marrow Mesenchymal Stem Cells Promote Tumor Growth Through Hedgehog Signaling Pathway, Cellular Physiology and Biochemistry, vol.42, issue.6, pp.2242-2254, 2017. ,
Adipose-derived mesenchymal stem cells promote osteosarcoma proliferation and metastasis by activating the STAT3 pathway, Oncotarget, vol.8, issue.14, pp.23803-23816, 2017. ,
The contribution of immune infiltrates and the local microenvironment in the pathogenesis of osteosarcoma, Cellular Immunology, vol.343, p.103711, 2019. ,
URL : https://hal.archives-ouvertes.fr/inserm-01644725
Tumor-Associated Macrophages: From Mechanisms to Therapy, Immunity, vol.41, issue.1, pp.49-61, 2014. ,
CD163-positive tumor-associated macrophages and CD8-positive cytotoxic lymphocytes are powerful diagnostic markers for the therapeutic stratification of osteosarcoma patients: An immunohistochemical analysis of the biopsies fromthe French OS2006 phase 3 trial, OncoImmunology, vol.6, issue.9, p.e1331193, 2017. ,
Tumor-Infiltrating Macrophages Are Associated with Metastasis Suppression in High-Grade Osteosarcoma: A Rationale for Treatment with Macrophage Activating Agents, Clinical Cancer Research, vol.17, issue.8, pp.2110-2119, 2011. ,
Dysregulation of macrophage polarization is associated with the metastatic process in osteosarcoma, Oncotarget, vol.7, issue.48, pp.78343-78354, 2016. ,
URL : https://hal.archives-ouvertes.fr/inserm-01466103
All-Trans Retinoic Acid Prevents Osteosarcoma Metastasis by Inhibiting M2 Polarization of Tumor-Associated Macrophages, Cancer Immunology Research, vol.5, issue.7, pp.547-559, 2017. ,
Macrophage Infiltration Predicts a Poor Prognosis for Human Ewing Sarcoma, The American Journal of Pathology, vol.179, issue.3, pp.1157-1170, 2011. ,
Clinicopathological correlation of tumor-associated macrophages in Ewing sarcoma, Biomedical Papers, vol.162, issue.1, pp.54-60, 2018. ,
CD163 + M2-type tumor-associated macrophage support the suppression of tumor-infiltrating T cells in osteosarcoma, International Immunopharmacology, vol.34, pp.101-106, 2016. ,
Metabolic Competition in the Tumor Microenvironment Is a Driver of Cancer Progression, Cell, vol.162, issue.6, pp.1229-1241, 2015. ,
A Feedback Loop between Hypoxia and Matrix Stress Relaxation Increases Oxygen-Axis Migration and Metastasis in Sarcoma, Cancer Research, vol.79, issue.8, pp.1981-1995, 2019. ,
TET2-dependent IL-6 induction mediated by the tumor microenvironment promotes tumor metastasis in osteosarcoma, Oncogene, vol.37, issue.22, pp.2903-2920, 2018. ,
The Ewing Sarcoma Secretome and Its Response to Activation of Wnt/beta-catenin Signaling, Molecular & Cellular Proteomics, vol.17, issue.5, pp.901-912, 2018. ,
Gene expression profiling of Ewing sarcoma tumours reveals the prognostic importance of tumour-stromal interactions: a report from the Children's Oncology Group, The Journal of Pathology: Clinical Research, vol.1, issue.2, pp.83-94, 2015. ,
Expression of CCL21 in Ewing sarcoma shows an inverse correlation with metastases and is a candidate target for immunotherapy, Cancer Immunology, Immunotherapy, vol.65, issue.8, pp.995-1002, 2016. ,
Zoledronate in combination with chemotherapy and surgery to treat osteosarcoma (OS2006): a randomised, multicentre, open-label, phase 3 trial, The Lancet Oncology, vol.17, issue.8, pp.1070-1080, 2016. ,
URL : https://hal.archives-ouvertes.fr/inserm-01702092
Clinical and prognostic significance of PD-1 and PD-L1 expression in sarcomas, Medical Oncology, vol.33, issue.8, 2016. ,
Enhanced T-Cell Immunity to Osteosarcoma Through Antibody Blockade of PD-1/PD-L1 Interactions, Journal of Immunotherapy, vol.38, issue.3, pp.96-106, 2015. ,
Programmed Cell Death Ligand 1 Expression in Osteosarcoma, Cancer Immunology Research, vol.2, issue.7, pp.690-698, 2014. ,
Increased PD-L1 and T-cell infiltration in the presence of HLA class I expression in metastatic high-grade osteosarcoma: a rationale for T-cell-based immunotherapy, Cancer Immunology, Immunotherapy, vol.66, issue.1, pp.119-128, 2016. ,
The Wnt signaling pathway in cancer, Critical Reviews in Oncology/Hematology, vol.99, pp.141-149, 2016. ,
Wnt Signaling in Cancer, Cold Spring Harbor Perspectives in Biology, vol.4, issue.5, pp.a008052-a008052, 2012. ,
Targeting the WNT Signaling Pathway in Cancer Therapeutics, The Oncologist, vol.20, issue.10, pp.1189-1198, 2015. ,
Wilms Tumor Suppressor WTX Negatively Regulates WNT/ -Catenin Signaling, Science, vol.316, issue.5827, pp.1043-1046, 2007. ,
YAP/TAZ Incorporation in the ?-Catenin Destruction Complex Orchestrates the Wnt Response, Cell, vol.158, issue.1, pp.157-170, 2014. ,
Wnt Stabilization of -Catenin Reveals Principles for Morphogen Receptor-Scaffold Assemblies, Science, vol.340, issue.6134, pp.867-870, 2013. ,
Control of ?-Catenin Phosphorylation/Degradation by a Dual-Kinase Mechanism, Cell, vol.108, issue.6, pp.837-847, 2002. ,
A partnership with the proteasome; the destructive nature of GSK3, Biochemical Pharmacology, vol.147, pp.77-92, 2018. ,
The -Catenin Destruction Complex, Cold Spring Harbor Perspectives in Biology, vol.5, issue.1, pp.a007898-a007898, 2012. ,
Molecular functions of the TLE tetramerization domain in Wnt target gene repression, The EMBO Journal, vol.33, issue.7, pp.719-731, 2014. ,
Wnt Signaling through Inhibition of ?-Catenin Degradation in an Intact Axin1 Complex, Cell, vol.149, issue.6, pp.1245-1256, 2012. ,
Wnt some lose some: transcriptional governance of stem cells by Wnt/ -catenin signaling, Genes & Development, vol.28, issue.14, pp.1517-1532, 2014. ,
Wnt/?-Catenin Signaling: Components, Mechanisms, and Diseases, Developmental Cell, vol.17, issue.1, pp.9-26, 2009. ,
WNT signaling in bone homeostasis and disease: from human mutations to treatments, Nature Medicine, vol.19, issue.2, pp.179-192, 2013. ,
The Wnt signaling pathway: a potential therapeutic target against cancer, Annals of the New York Academy of Sciences, vol.1443, issue.1, pp.54-74, 2019. ,
Wnt signaling in cancer, Oncogene, vol.36, issue.11, pp.1461-1473, 2016. ,
Screening for natural products that affect Wnt signaling activity, Journal of Natural Medicines, vol.73, issue.4, pp.697-705, 2019. ,
Targeting the Wnt pathway in human cancers: Therapeutic targeting with a focus on OMP-54F28, Pharmacology & Therapeutics, vol.146, pp.1-11, 2015. ,
Targeting the Wnt Pathway in Cancer: A Review of Novel Therapeutics, Targeted Oncology, vol.12, issue.5, pp.623-641, 2017. ,
Modulating the wnt signaling pathway with small molecules, Protein Science, vol.26, issue.4, pp.650-661, 2017. ,
Development of anticancer agents targeting the Wnt/?-catenin signaling, Am. J. Cancer Res, vol.5, pp.2344-2360, 2015. ,
Recent Development of Wnt Signaling Pathway Inhibitors for Cancer Therapeutics, Current Oncology Reports, vol.21, issue.2, 2019. ,
Targeting the Wnt/beta-catenin pathway in cancer: Update on effectors and inhibitors, Cancer Treatment Reviews, vol.62, pp.50-60, 2018. ,
Can we safely target the WNT pathway?, Nature Reviews Drug Discovery, vol.13, issue.7, pp.513-532, 2014. ,
Aberrant activation of Wnt/?-catenin signaling drives proliferation of bone sarcoma cells, Oncotarget, vol.6, issue.19, pp.17570-17583, 2015. ,
Cytoplasmic and/or nuclear staining of beta-catenin is associated with lung metastasis, Clinical and Experimental Metastasis, vol.20, issue.6, pp.525-529, 2003. ,
Dioscin inhibits stem-cell-like properties and tumor growth of osteosarcoma through Akt/GSK3/?-catenin signaling pathway, Cell Death & Disease, vol.9, issue.3, p.343, 2018. ,
Aberrant CXCR4 and ?-catenin expression in osteosarcoma correlates with patient survival, Oncology Letters, vol.10, issue.4, pp.2123-2129, 2015. ,
Inactive Wnt/β-catenin pathway in conventional high-grade osteosarcoma, The Journal of Pathology, vol.220, issue.1, pp.24-33, 2010. ,
Profiling of high-grade central osteosarcoma and its putative progenitor cells identifies tumourigenic pathways, British Journal of Cancer, vol.101, issue.11, pp.1909-1918, 2009. ,
The genetic basis for inactivation of Wnt pathway in human osteosarcoma, BMC Cancer, vol.14, issue.1, 2014. ,
Efficacy of glycogen synthase kinase-3? targeting against osteosarcoma via activation of ?-catenin, Oncotarget, vol.7, issue.47, pp.77038-77051, 2016. ,
Epithelial-to-mesenchymal transition, circulating tumor cells and cancer metastasis: Mechanisms and clinical applications, Oncotarget, vol.8, issue.46, pp.81558-81571, 2017. ,
Expression profile of Twist, vascular endothelial growth factor and CD34 in patients with different phases of osteosarcoma, Oncology Letters, vol.10, issue.1, pp.417-421, 2015. ,
Expression of Snail2 in long bone osteosarcomas correlates with tumour malignancy, Tumor Biology, vol.32, issue.3, pp.515-526, 2011. ,
Overexpression of ZEB1 relates to metastasis and invasion in osteosarcoma, Journal of Surgical Oncology, vol.105, issue.8, pp.830-834, 2011. ,
EMT transcription factors: implication in osteosarcoma, Medical Oncology, vol.30, issue.4, 2013. ,
Transforming Growth Factor-? Signaling Plays a Pivotal Role in the Interplay Between Osteosarcoma Cells and Their Microenvironment, Frontiers in Oncology, vol.8, p.133, 2018. ,
URL : https://hal.archives-ouvertes.fr/inserm-01814333
Transcriptional crosstalk between TGF? and stem cell pathways in tumor cell invasion: Role of EMT promoting Smad complexes, Cell Cycle, vol.9, issue.12, pp.2363-2374, 2010. ,
Bone morphogenetic protein?2 promotes osteosarcoma growth by promoting epithelial?mesenchymal transition (EMT) through the Wnt/??catenin signaling pathway, Journal of Orthopaedic Research, vol.37, issue.7, pp.1638-1648, 2019. ,
Fibulin-3 promotes osteosarcoma invasion and metastasis by inducing epithelial to mesenchymal transition and activating the Wnt/?-catenin signaling pathway, Scientific Reports, vol.7, issue.1, 2017. ,
Carboxypeptidase E-?N promotes migration, invasiveness, and epithelial?mesenchymal transition of human osteosarcoma cells via the Wnt??-catenin pathway, Biochemistry and Cell Biology, vol.97, issue.4, pp.446-453, 2019. ,
Ubiquitin?specific protease 7 promotes osteosarcoma cell metastasis by inducing epithelial?mesenchymal transition, Oncology Reports, vol.41, pp.543-551, 2018. ,
Signalling mechanism(s) of epithelial?mesenchymal transition and cancer stem cells in tumour therapeutic resistance, Clinica Chimica Acta, vol.483, pp.156-163, 2018. ,
Targeting Notch, Hedgehog, and Wnt pathways in cancer stem cells: clinical update, Nature Reviews Clinical Oncology, vol.12, issue.8, pp.445-464, 2015. ,
CD117 and Stro-1 Identify Osteosarcoma Tumor-Initiating Cells Associated with Metastasis and Drug Resistance, Cancer Research, vol.70, issue.11, pp.4602-4612, 2010. ,
Targeting the osteosarcoma cancer stem cell, Journal of Orthopaedic Surgery and Research, vol.5, issue.1, p.78, 2010. ,
Osteosarcoma Stem Cells Have Active Wnt/?-catenin and Overexpress SOX2 and KLF4, Journal of Cellular Physiology, vol.231, issue.4, pp.876-886, 2015. ,
miR?552?5p facilitates osteosarcoma cell proliferation and metastasis by targeting WIF1, Experimental and Therapeutic Medicine, vol.17, pp.3781-3788, 2019. ,
MicroRNA?873 targets HOXA9 to inhibit the aggressive phenotype of osteosarcoma by deactivating the Wnt/??catenin pathway, International Journal of Oncology, vol.54, pp.1809-1820, 2019. ,
miR?885?5p suppresses osteosarcoma proliferation, migration and invasion through regulation of ??catenin, Oncology Letters, vol.17, 2018. ,
microRNA-758 inhibits the malignant phenotype of osteosarcoma cells by directly targeting HMGA1 and deactivating the Wnt/?-catenin pathway, Am. J. Cancer Res, vol.9, pp.36-52, 2019. ,
MicroRNA?377 exerts a potent suppressive role in osteosarcoma through the involvement of the histone acetyltransferase 1?mediated Wnt axis, Journal of Cellular Physiology, vol.234, issue.12, pp.22787-22798, 2019. ,
LncRNA AWPPH promotes osteosarcoma progression via activation of Wnt/?-catenin pathway through modulating miR-93-3p/FZD7 axis, Biochemical and Biophysical Research Communications, vol.514, issue.3, pp.1017-1022, 2019. ,
Wnt Signaling in Osteosarcoma, Advances in Experimental Medicine and Biology, vol.804, pp.33-45, 2014. ,
Wnt Signaling in Ewing Sarcoma, Osteosarcoma, and Malignant Peripheral Nerve Sheath Tumors, Current Osteoporosis Reports, vol.15, issue.4, pp.239-246, 2017. ,
Activation of Wnt/ -Catenin in Ewing Sarcoma Cells Antagonizes EWS/ETS Function and Promotes Phenotypic Transition to More Metastatic Cell States, Cancer Research, vol.76, issue.17, pp.5040-5053, 2016. ,
LGR5 is Expressed by Ewing Sarcoma and Potentiates Wnt/?-Catenin Signaling, Frontiers in Oncology, vol.3, 2013. ,
Wnt/Frizzled signaling in Ewing sarcoma, Pediatr. Blood Cancer, vol.43, pp.243-249, 2004. ,
Wnt-3a and Dickkopf-1 Stimulate Neurite Outgrowth in Ewing Tumor Cells via a Frizzled3- and c-Jun N-Terminal Kinase-Dependent Mechanism, Molecular and Cellular Biology, vol.28, issue.7, pp.2368-2379, 2008. ,
The EWS/FLI1 oncogenic protein inhibits expression of the Wnt inhibitor DICKKOPF-1 gene and antagonizes ?-catenin/TCF-mediated transcription, Carcinogenesis, vol.31, issue.3, pp.394-401, 2009. ,
Inhibition of porcupine prolongs metastasis free survival in a mouse xenograft model of Ewing sarcoma, Oncotarget, vol.8, issue.45, pp.78265-78276, 2017. ,
Biology of Bone Sarcomas and New Therapeutic Developments, Calcified Tissue International, vol.102, issue.2, pp.174-195, 2017. ,
URL : https://hal.archives-ouvertes.fr/inserm-01653600
Regulation of bone formation by osteoclasts involves Wnt/BMP signaling and the chemokine sphingosine-1-phosphate, Proceedings of the National Academy of Sciences, vol.105, issue.52, pp.20764-20769, 2008. ,
Wnt signaling in osteoblasts and bone diseases, Gene, vol.341, pp.19-39, 2004. ,
Wntless functions in mature osteoblasts to regulate bone mass, Proceedings of the National Academy of Sciences, vol.109, issue.33, pp.E2197-E2204, 2012. ,
Wnt Signaling Inhibits Osteoclast Differentiation by Activating Canonical and Noncanonical cAMP/PKA Pathways, Journal of Bone and Mineral Research, vol.31, issue.1, pp.65-75, 2015. ,
A Cross-Species Analysis of a Mouse Model of Breast Cancer-Specific Osteolysis and Human Bone Metastases Using Gene Expression Profiling, BMC Cancer, vol.11, issue.1, 2011. ,
Breast cancer?derived Dickkopf1 inhibits osteoblast differentiation and osteoprotegerin expression: Implication for breast cancer osteolytic bone metastases, International Journal of Cancer, vol.123, issue.5, pp.1034-1042, 2008. ,
Matrix Metalloproteinases Participate in Osteosarcoma Invasion, Journal of Surgical Research, vol.127, issue.2, pp.151-156, 2005. ,
Elevated ratio of MMP2/MMP9 activity is associated with poor response to chemotherapy in osteosarcoma, BMC Cancer, vol.16, issue.1, 2016. ,
Association of MMP-2 expression and prognosis in osteosarcoma patients, Int. J. Clin. Exp. Pathol, vol.8, pp.14965-14970, 2015. ,
Prognostic significance of matrix metalloproteinase 9 expression in osteosarcoma, Medicine, vol.97, issue.44, p.e13051, 2018. ,
Protein expression of matrix metalloproteinase (MMP-1, -2, -3, -9 and -14) in Ewing family tumors and medulloblastomas of pediatric patients, J. Pediatr. Genet, vol.1, pp.181-187, 2012. ,
Beclin-1 knockdown decreases proliferation, invasion and migration of Ewing sarcoma SK-ES-1 cells via inhibition of MMP-9, Oncology Letters, vol.15, pp.3221-3225, 2017. ,
Blocking Wnt/LRP5 signaling by a soluble receptor modulates the epithelial to mesenchymal transition and suppresses met and metalloproteinases in osteosarcoma Saos-2 cells, Journal of Orthopaedic Research, vol.25, issue.7, pp.964-971, 2007. ,
A furin inhibitor downregulates osteosarcoma cell migration by downregulating the expression levels of MT1-MMP via the Wnt signaling pathway, Oncology Letters, vol.7, issue.4, pp.1033-1038, 2014. ,
Influence of ?-catenin small interfering RNA on human osteosarcoma cells, Journal of Huazhong University of Science and Technology [Medical Sciences], vol.31, issue.3, pp.353-358, 2011. ,
SiRNA-mediated silencing of beta-catenin suppresses invasion and chemosensitivity to doxorubicin in MG-63 osteosarcoma cells. Asian Pac, J. Cancer Prev, vol.12, pp.239-245, 2011. ,
Tenascin C in metastasis: A view from the invasive front, Cell Adhesion & Migration, vol.9, issue.1-2, pp.112-124, 2015. ,
The Role of Wnt Signalling in Angiogenesis, Clin. Biochem. Rev, vol.38, pp.131-142, 2017. ,
Principles and mechanisms of vessel normalization for cancer and other angiogenic diseases, Nature Reviews Drug Discovery, vol.10, issue.6, pp.417-427, 2011. ,
Neovascularization in Ewing?s sarcoma, Neoplasma, vol.65, issue.03, pp.317-325, 2018. ,
A systematic review of vascular endothelial growth factor expression as a biomarker of prognosis in patients with osteosarcoma, Tumor Biology, vol.34, issue.3, pp.1895-1899, 2013. ,
Genetic amplification of the vascular endothelial growth factor (VEGF) pathway genes, includingVEGFA, in human osteosarcoma, Cancer, vol.117, issue.21, pp.4925-4938, 2011. ,
Vascular Endothelial Growth Factor Expression is Up-Regulated by EWS-ETS Oncoproteins and Sp1 and May Represent an Independent Predictor of Survival in Ewing's Sarcoma, Clinical Cancer Research, vol.10, issue.4, pp.1344-1353, 2004. ,
Tumor Cell Plasticity in Ewing Sarcoma, an Alternative Circulatory System Stimulated by Hypoxia, Cancer Research, vol.65, issue.24, pp.11520-11528, 2005. ,
Bone Marrow Subsets Differentiate into Endothelial Cells and Pericytes Contributing to Ewing's Tumor Vessels, Molecular Cancer Research, vol.6, issue.6, pp.929-936, 2008. ,
VEGF165 expression in the tumor microenvironment influences the differentiation of bone marrow-derived pericytes that contribute to the Ewing?s sarcoma vasculature, Angiogenesis, vol.11, issue.3, pp.257-267, 2008. ,
beta-Catenin regulates vascular endothelial growth factor expression in colon cancer, Cancer Res, vol.63, pp.3145-3153, 2003. ,
VEGF is regulated by the WNT pathway in colon cancer, Gastroenterology, vol.120, issue.5, pp.A4-A4, 2001. ,
Secreted antagonists of the Wnt signalling pathway, Journal of Cell Science, vol.116, issue.13, pp.2627-2634, 2003. ,
Regulation of Endothelial Cell Cytoskeletal Reorganization by a Secreted Frizzled-Related Protein-1 and Frizzled 4- and Frizzled 7-Dependent Pathway, The American Journal of Pathology, vol.172, issue.1, pp.37-49, 2008. ,
URL : https://hal.archives-ouvertes.fr/inserm-00508525
Identification and cloning of a secreted protein related to the cysteine-rich domain of frizzled. Evidence for a role in endothelial cell growth control, Circ. Res, vol.84, pp.1433-1445, 1999. ,
Secreted Frizzled-Related Protein 4, The American Journal of Pathology, vol.176, issue.3, pp.1505-1516, 2010. ,
NKD2, a negative regulator of Wnt signaling, suppresses tumor growth and metastasis in osteosarcoma, Oncogene, vol.34, issue.39, pp.5069-5079, 2015. ,
Hypoxia: A key regulator of angiogenesis in cancer, Cancer and Metastasis Reviews, vol.26, issue.2, pp.281-290, 2007. ,
Activation of vascular endothelial growth factor gene transcription by hypoxia-inducible factor 1., Molecular and Cellular Biology, vol.16, issue.9, pp.4604-4613, 1996. ,
Hypoxia-Induced Angiogenesis: Good and Evil, Genes & Cancer, vol.2, issue.12, pp.1117-1133, 2011. ,
Hypoxia, stem cells and bone tumor, Cancer Letters, vol.313, issue.2, pp.129-136, 2011. ,
Effects of siRNA-mediated HIF-1? gene silencing on angiogenesis in osteosarcoma, Pakistan Journal of Medical Sciences, vol.33, issue.2, pp.341-346, 2017. ,
Hypoxia Modulates EWS-FLI1 Transcriptional Signature and Enhances the Malignant Properties of Ewing's Sarcoma Cells In vitro, Cancer Research, vol.70, issue.10, pp.4015-4023, 2010. ,
Hypoxia promotes osteosarcoma cell proliferation and migration through enhancing platelet-derived growth factor-BB/platelet-derived growth factor receptor-? axis, Biochemical and Biophysical Research Communications, vol.512, issue.2, pp.360-366, 2019. ,
Hypoxia-inducible factor-1 promotes cancer progression through activating AKT/Cyclin D1 signaling pathway in osteosarcoma, Biomedicine & Pharmacotherapy, vol.105, pp.1-9, 2018. ,
Prognosis value of Hypoxia-inducible factor-1? expression in patients with bone and soft tissue sarcoma: a meta-analysis, SpringerPlus, vol.5, issue.1, 2016. ,
BMPR2 and HIF1-? overexpression in resected osteosarcoma correlates with distant metastasis and patient survival, Chinese Journal of Cancer Research, vol.29, issue.5, pp.447-454, 2017. ,
Prognostic role of hypoxia-inducible factor-1 alpha expression in osteosarcoma: a meta-analysis, OncoTargets and Therapy, vol.9, p.1477, 2016. ,
Malignant progression of invasive tumour cells seen in hypoxia present an accumulation of ?-catenin in the nucleus at the tumour front, Experimental and Molecular Pathology, vol.87, issue.2, pp.109-116, 2009. ,
Interaction between ?-catenin and HIF-1 promotes cellular adaptation to hypoxia, Nature Cell Biology, vol.9, issue.2, pp.210-217, 2007. ,
Down Regulation of Wnt Signaling Mitigates Hypoxia-Induced Chemoresistance in Human Osteosarcoma Cells, PLoS ONE, vol.9, issue.10, p.e111431, 2014. ,
Exploiting Signaling Pathways and Immune Targets Beyond the Standard of Care for Ewing Sarcoma, Frontiers in Oncology, vol.9, 2019. ,
Macrophage Diversity Enhances Tumor Progression and Metastasis, Cell, vol.141, issue.1, pp.39-51, 2010. ,
Crosstalk between hepatic tumor cells and macrophages via Wnt/?-catenin signaling promotes M2-like macrophage polarization and reinforces tumor malignant behaviors, Cell Death & Disease, vol.9, issue.8, p.793, 2018. ,
Pre-operative lymphocyte-to-monocyte ratio as a predictor of overall survival in patients suffering from osteosarcoma, FEBS Open Bio, vol.5, issue.1, pp.682-687, 2015. ,
Emerging targets in cancer immunotherapy: beyond CTLA-4 and PD-1, Immunotherapy, vol.7, issue.11, pp.1169-1186, 2015. ,
HHLA2, a member of the B7 family, is expressed in human osteosarcoma and is associated with metastases and worse survival, Scientific Reports, vol.6, issue.1, 2016. ,
Immune infiltration and PD-L1 expression in the tumor microenvironment are prognostic in osteosarcoma, Scientific Reports, vol.6, issue.1, 2016. ,
Programmed cell death ligand 1 (PD-L1) expression is not a predominant feature in Ewing sarcomas, Pediatric Blood & Cancer, vol.65, issue.1, p.e26719, 2017. ,
Programmed cell death-1 blockade in recurrent disseminated Ewing sarcoma, Journal of Hematology & Oncology, vol.9, issue.1, p.48, 2016. ,
B7-H3 is Overexpressed in Patients Suffering Osteosarcoma and Associated with Tumor Aggressiveness and Metastasis, PLoS ONE, vol.8, issue.8, p.e70689, 2013. ,
Expression of B7-H3 in cancer tissue during osteosarcoma progression in nude mice, Genetics and Molecular Research, vol.14, issue.4, pp.14253-14261, 2015. ,
A Review of the Role of Wnt in Cancer Immunomodulation, Cancers, vol.11, issue.6, p.771, 2019. ,
WNT/?-catenin Pathway Activation Correlates with Immune Exclusion across Human Cancers, Clinical Cancer Research, vol.25, issue.10, pp.3074-3083, 2019. ,
Melanoma-intrinsic ?-catenin signalling prevents anti-tumour immunity, Nature, vol.523, issue.7559, pp.231-235, 2015. ,
?-Catenin Activation Promotes Immune Escape and Resistance to Anti?PD-1 Therapy in Hepatocellular Carcinoma, Cancer Discovery, vol.9, issue.8, pp.1124-1141, 2019. ,
Anti-PD-1 therapy redirects macrophages from an M2 to an M1 phenotype inducing regression of OS lung metastases, Cancer Medicine, vol.7, issue.6, pp.2654-2664, 2018. ,
WNT Signaling in Cancer Immunosurveillance, Trends in Cell Biology, vol.29, issue.1, pp.44-65, 2019. ,
, Figure 2?figure supplement 1. Representative images used in the experiment, sampled from http://commons.wikimedia.org/wiki/Main_Page under the Creative Commons Attribution 4.0 International Public License https://creativecommons.org/licenses/by/4.0/., © 2019 by the authors. Licensee MDPI