Highly sensitive quantification of plasma SARS-CoV-2 RNA shelds light on its potential clinical value - Archive ouverte HAL Access content directly
Journal Articles Clinical Infectious Diseases Year : 2020

Highly sensitive quantification of plasma SARS-CoV-2 RNA shelds light on its potential clinical value

(1, 2) , (3) , (2) , (1, 4) , (1) , (2) , (2) , (2) , (5, 1) , (1) , (1, 6) , (6) , (1, 7)
1
2
3
4
5
6
7
Valérie Taly
Flore Rozenberg

Abstract

Background. Coronavirus disease 2019 (COVID-19) is a global public health problem that has already caused more than 662,000 deaths worldwide. Although the clinical manifestations of COVID-19 are dominated by respiratory symptoms, some patients present other severe damage such as cardiovascular, renal and liver injury or/and multiple organ failure, suggesting a spread of the SARS-CoV-2 in blood. Recent ultrasensitive polymerase chain reaction (PCR) technology now allows absolute quantification of nucleic acids in plasma. We herein intended to use the droplet-based digital PCR technology to obtain sensitive detection and precise quantification of plasma SARS-CoV-2 viral load (SARSCoV-2 RNAaemia) in hospitalized COVID-19 patients.Methods. Fifty-eight consecutive COVID-19 patients with pneumonia 8 to 12 days after onset of symptoms and 12 healthy controls were analyzed. Disease severity was categorized as mild-to-moderate in 17 patients, severe in 16 patients and critical in 26 patients. Plasma SARS-CoV-2 RNAaemia was quantified by droplet digital Crystal Digital PCR™ nextgeneration technology (Stilla Technologies, Villejuif, France).Results. Overall, SARS-CoV-2 RNAaemia was detected in 43 (74.1%) patients. Prevalence of positive SARS-CoV-2 RNAaemia correlated with disease severity, ranging from 53% in mild-to-moderate patients to 88% in critically ill patients (p=0.036). Levels of SARS-CoV-2 RNAaemia were associated with severity (p=0.035). Among nine patients who experienced clinical deterioration during follow-up, eight had positive SARS-CoV-2 RNAaemia at baseline while only one critical patient with undetectable SARS-CoV-2 RNAaemia at thetime of analysis died at day 27.Conclusion. SARS-CoV-2 RNAaemia measured by droplet-based digital PCR constitutes a promising prognosis biomarker in COVID-19 patients.
Embargoed file
Embargoed file
Ne sera jamais visible
Loading...

Dates and versions

inserm-02992971 , version 1 (06-11-2020)

Identifiers

Cite

David Veyer, Solen Kernéis, Geoffroy Poulet, Maxime Wack, Nicolas Robillard, et al.. Highly sensitive quantification of plasma SARS-CoV-2 RNA shelds light on its potential clinical value. Clinical Infectious Diseases, 2020, pp.ciaa1196. ⟨10.1093/cid/ciaa1196⟩. ⟨inserm-02992971⟩
102 View
1 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More