Skip to Main content Skip to Navigation
Journal articles

Emerging isothermal amplification technologies for microRNA biosensing: Applications to liquid biopsies

Abstract : The potential of microRNAs (miRNAs) as biomarker candidates in clinical practice for diagnosis, prognosis and treatment response prediction, especially in liquid biopsies, has led to a tremendous demand for techniques that can detect these molecules rapidly and accurately. Hence, numerous achievements have been reported recently in miRNA research. In this review, we discuss the challenges associated with the emerging field of miRNA detection, which are linked to the intrinsic properties of miRNAs, advantages and drawbacks of the currently available technologies and their potential applications in clinical research. We summarize the most promising nucleic acid amplification techniques applied to the in vitro detection of miRNAs, with a particular emphasis on the state of the art for isothermal alternatives to RT-qPCR. We detail the sensitivity, specificity and quantitativity of these approaches, as well as their potential for multiplexing. We also review the different detection formats to which these chemistries have been adapted, including analog readouts such as real-time monitoring, digital counting based on single-molecule amplification in compartments, and surface-based strategies.
Document type :
Journal articles
Complete list of metadata

Cited literature [240 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-02992632
Contributor : Valerie Taly Connect in order to contact the contributor
Submitted on : Tuesday, November 10, 2020 - 10:05:23 AM
Last modification on : Tuesday, October 19, 2021 - 11:15:18 PM
Long-term archiving on: : Friday, February 12, 2021 - 12:14:37 PM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

Citation

Guillaume Gines, Roberta Menezes, Wenjin Xiao, Yannick Rondelez, Valérie Taly. Emerging isothermal amplification technologies for microRNA biosensing: Applications to liquid biopsies. Molecular Aspects of Medicine, Elsevier, 2020, 72, pp.100832. ⟨10.1016/j.mam.2019.11.002⟩. ⟨inserm-02992632⟩

Share

Metrics

Record views

72