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Green and Tunable Animal Protein-Free Microcarriers for Cell Expansion

Abstract : Cell culture on microcarriers emerges as an alternative of two-dimensional culture to produce large cell doses, which are required for cell-based therapies. Herein, we report a versatile and easy solvent-free greener fabrication process to prepare microcarriers based on a biosourced and compostable polymer. The preparation of the microcarrier core, which is based on poly(L-lactide) crystallization from a polymer blend, allows us to easily tune the density, porosity, and size of the microparticles. A bioadhesive coating based on biopolymers, devoid of animal protein and optimized to improve cell adhesion, is then successfully deposited on the surface of the microcarriers. The ability of these new microcarriers to expand human adipose-derived stromal cells with good yield, in semistatic and dynamic conditions, is demonstrated. Finally, bead-to-bead cell transfer is shown to increase the yield of cell production without having to stop the culture. These microcarriers are therefore a promising and efficient green alternative to currently existing systems.
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Contributor : Arnaud Eyraud Connect in order to contact the contributor
Submitted on : Friday, October 30, 2020 - 2:24:34 PM
Last modification on : Wednesday, April 27, 2022 - 4:02:54 AM




Eleana Somville, Anitha Ajith Kumar, Jérôme Guicheux, Boris Halgand, Sophie Demoustier-Champagne, et al.. Green and Tunable Animal Protein-Free Microcarriers for Cell Expansion. ACS Applied Materials & Interfaces, Washington, D.C. : American Chemical Society, 2020, Online ahead of print. ⟨10.1021/acsami.0c16875⟩. ⟨inserm-02983932⟩



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