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, Tf-Alexa 488 (left) co-localized (right) with Müller glial cells stained with anti-cellular retinaldehyde-binding protein (CRALBP) antibody (middle). (B-C) Higher magnification revealed Tf-Alexa on apical side of retinal pigment epithelium revealed with anti-RPE65 antibody (red) (B), and at photoreceptors segments layers stained with rhodopsin (red, asterisk) (C). (D) Tf-Alexa merged (yellow) with transferrin receptor 1 (red), Alexa 488 (green). (A) Two hours after injection

, Alexa co-localized with transferrin receptor 2 (red) in inner segments, outer nuclear layer and inner plexiform layer. (F) Six hours after injection, Tf-Alexa was in overall retina and additionally detected in choroid (Ch) vessels. (G-H) Transferrin levels were quantified in posterior segment tissues (G) and ocular fluids (H) by ELISA assay, 6 and 24 hours after Tf injection. All values are represented as mean± SEM. Student's t-test

*. , , p.1

;. Ch:-choroid and . Gcl, Ganglion cell layer; INL: Inner nuclear layer; IPL: Inner plexiform layer; IS: Inner segment; ONL: Outer nuclear layer; OPL: Outer plexiform layer; OS: Outer segment; RPE: retinal pigment epithelium

, Figure 2: IVT injections of transferrin have no retinal toxicity

, In vivo retinal structure recorded by optical coherence tomography (OCT) 7 days after first transferrin (Tf) IVT injection (D7) and 7 days after the second Tf injection (D14) in rats, demonstrated no retinal histological modifications. (B) Measurement of outer nuclear layer (ONL) from OCT pictures in control not injected rats and rats