S. Charrin, S. Jouannet, C. Boucheix, and E. Rubinstein, Tetraspanins at a glance, J. Cell Sci, vol.127, pp.3641-3648, 2014.

M. S. Pols and J. Klumperman, Trafficking and function of the tetraspanin CD63, Exp. Cell Res, vol.315, pp.1584-1592, 2009.

T. Yoshida, Y. Kawano, K. Sato, Y. Ando, J. Aoki et al., A CD63 mutant inhibits T-cell tropic human immunodeficiency virus type 1 entry by disrupting CXCR4 trafficking to the plasma membrane, Traffic, vol.9, pp.540-558, 2008.

A. Duffield, E. J. Kamsteeg, A. N. Brown, P. Pagel, and M. J. Caplan, The tetraspanin CD63 enhances the internalization of the H,K-ATPase beta-subunit, Proc. Natl. Acad. Sci, vol.100, pp.15560-15565, 2003.

J. R. Edgar, E. R. Eden, and C. E. Futter, Hrs-and CD63-dependent competing mechanisms make different sized endosomal intraluminal vesicles, Traffic, vol.15, pp.197-211, 2014.

S. Kraft, M. H. Jouvin, N. Kulkarni, S. Kissing, E. S. Morgan et al., The tetraspanin CD63 is required for efficient IgE-mediated mast cell degranulation and anaphylaxis, J. Immunol, vol.191, pp.2871-2878, 2013.

G. Li, M. A. Endsley, A. Somasunderam, S. L. Gbota, M. I. Mbaka et al., The dual role of tetraspanin CD63 in HIV-1 replication, Virol. J, vol.11, issue.23, 2014.

L. Gräßel, L. A. Fast, K. D. Scheffer, F. Boukhallouk, G. A. Spoden et al., The CD63-Syntenin-1 Complex Controls Post-Endocytic Trafficking of Oncogenic Human Papillomaviruses, Sci. Rep, vol.6, 2016.

G. Van-niel, S. Charrin, S. Simoes, M. Romao, L. Rochin et al., The tetraspanin CD63 regulates ESCRT-independent and -dependent endosomal sorting during melanogenesis, Dev. Cell, vol.21, pp.708-721, 2011.

J. Schröder, R. Lüllmann-rauch, N. Himmerkus, I. Pleines, B. Nieswandt et al., Deficiency of the tetraspanin CD63 associated with kidney pathology but normal lysosomal function, Mol. Cell. Biol, vol.29, pp.1083-1094, 2009.

C. Boucheix and E. Rubinstein, Tetraspanins. Cell. Mol. Life Sci. CMLS, vol.58, pp.1189-1205, 2001.

Z. Andreu and M. Yáñez-mó, Tetraspanins in extracellular vesicle formation and function, Front. Immunol, vol.5, 2014.

G. Van-niel, G. Angelo, and G. Raposo, Shedding light on the cell biology of extracellular vesicles, Nat. Rev. Mol. Cell Biol, vol.19, pp.213-228, 2018.
URL : https://hal.archives-ouvertes.fr/hal-02359760

C. F. Manning, A. M. Bundros, and J. S. Trimmer, Benefits and pitfalls of secondary antibodies: Why choosing the right secondary is of primary importance, PLoS ONE, vol.7, 2012.

J. Stavnezer and C. E. Schrader, IgH chain class switch recombination: Mechanism and regulation, J. Immunol, vol.193, pp.5370-5378, 2014.

C. E. Müller and K. Rajewsky, Isolation of immunoglobulin class switch variants from hybridoma lines secreting anti-idiotope antibodies by sequential sublining, J. Immunol, vol.131, pp.877-881, 1983.

A. Radbruch, B. Liesegang, and K. Rajewsky, Isolation of variants of mouse myeloma X63 that express changed immunoglobulin class, Proc. Natl. Acad. Sci, vol.77, pp.2909-2913, 1980.

G. Spira, P. Gregor, H. L. Aguila, and M. D. Scharff, Clonal variants of hybridoma cells that switch isotype at a high frequency, Proc. Natl. Acad. Sci, vol.91, pp.3423-3427, 1994.

S. Sen, W. S. Hu, and F. Srienc, Flow cytometric study of hybridoma cell culture: Correlation between cell surface fluorescence and IgG production rate, Enzym. Microb. Technol, vol.12, pp.571-576, 1990.

J. L. Dangl, D. R. Parks, V. T. Oi, and L. A. Herzenberg, Rapid isolation of cloned isotype switch variants using fluorescence activated cell sorting, Cytometry, vol.2, pp.395-401, 1982.

S. Charrin, F. Le-naour, M. Oualid, M. Billard, G. Faure et al., The major CD9 and CD81 molecular partner. Identification and characterization of the complexes, J. Biol. Chem, vol.276, pp.14329-14337, 2001.

C. Théry, S. Amigorena, G. Raposo, and A. Clayton, Isolation and characterization of exosomes from cell culture supernatants and biological fluids, Curr. Protoc. Cell Biol, 2006.

G. Raposo, D. Tenza, D. M. Murphy, J. F. Berson, and M. S. Marks, Distinct protein sorting and localization to premelanosomes, melanosomes, and lysosomes in pigmented melanocytic cells, J. Cell Biol, vol.152, pp.809-824, 2001.

G. Van-niel, P. Bergam, A. Di-cicco, I. Hurbain, A. Lo-cicero et al., Apolipoprotein E Regulates Amyloid Formation within Endosomes of Pigment Cells. Cell Rep, vol.13, pp.43-51, 2015.

J. Kowal, G. Arras, M. Colombo, M. Jouve, J. P. Morath et al., Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes, Proc. Natl. Acad. Sci, vol.113, pp.968-977, 2016.
URL : https://hal.archives-ouvertes.fr/inserm-02450678

M. Colombo, C. Moita, G. Van-niel, J. Kowal, J. Vigneron et al., Analysis of ESCRT functions in exosome biogenesis, composition and secretion highlights the heterogeneity of extracellular vesicles, J. Cell Sci, vol.126, pp.5553-5565, 2013.

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