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, Figure 6: Rescue of action potential rate in Cav1.3 -/-sinus node cells by MCaE12A

, Mouse sinus node (SAN) cells were isolated as described under "Material and methods

, Left panels; living SAN cells were incubated in presence of 1 µM Cy5-MCaE12A (cyan, p.15

, min a 37°C and washed twice with Tyrode. Cells were then fixed, permeabilized and 667 incubated over night at 4°C in presence of antibodies directed against HCN4 (green, p.668

, Right panel; fluorescence intensity of HCN4-Ab (red line), p.669

, While HCN4 is preferentially localized at the plasma membrane, Cy5-MCaE12A was 671 present within the cytoplasm. B; Action potential frequency in isolated SAN cells was 672 measured using current clamp configuration in absence (vehicle) and presence of 1 mM 673, Cy5-MCaE12A (black line) was measured along the dashed line shown on the images on left 670 panels

. Mcae12a, For Wt mice SAN cells * p = 0.025 (paired t test

. Cav1, 3 -/-SAN cells ** p = 0.011 (paired t test, n = 8 cells from 3 mice) and for Cav1

*. , , pp.10-13