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Paradoxical effect of 516 (left panel, control) and presence of MCaE12A (right panel, + MCaE12A).. C; left and middle 651 panel: statistical analysis of SD1 and SD2 values measured on Poincaré plots, p.652 ,
, ); right panel: Average interbeat interval was measured over a 30 653 seconds period before and after infusion of MCaE12A. D; spatiotemporal parameters of hiPS-654, pp.10-14
, MCaE12A modifies spatial dynamics of Ca 2+ sparks in isolated hiPS-CMs, Figure, vol.5
, A; representative recording of Ca 2+ sparks in isolated hiPS-CMs in absence (control) or 658 presence (+MCaE12A) of 1 µM MCaE12A. B; statistical analysis of the spatio-temporal 659 parameters of Ca 2+ sparks recorded in absence (control, opened circles) or presence 660 (+MCaE12A, filled circles) of 1 µM MCaE12A, (n=39 and 21 cells for control and MCaE12A 661 respectively
, Figure 6: Rescue of action potential rate in Cav1.3 -/-sinus node cells by MCaE12A
, Mouse sinus node (SAN) cells were isolated as described under "Material and methods
, Left panels; living SAN cells were incubated in presence of 1 µM Cy5-MCaE12A (cyan, p.15
, min a 37°C and washed twice with Tyrode. Cells were then fixed, permeabilized and 667 incubated over night at 4°C in presence of antibodies directed against HCN4 (green, p.668
, Right panel; fluorescence intensity of HCN4-Ab (red line), p.669
, While HCN4 is preferentially localized at the plasma membrane, Cy5-MCaE12A was 671 present within the cytoplasm. B; Action potential frequency in isolated SAN cells was 672 measured using current clamp configuration in absence (vehicle) and presence of 1 mM 673, Cy5-MCaE12A (black line) was measured along the dashed line shown on the images on left 670 panels
For Wt mice SAN cells * p = 0.025 (paired t test ,
3 -/-SAN cells ** p = 0.011 (paired t test, n = 8 cells from 3 mice) and for Cav1 ,
, , pp.10-13