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The macrophage microtubule network acts as a key cellular controller of the intracellular fate of Leishmania infantum

Abstract : The parasitophorous vacuoles (PVs) that insulate Leishmania spp. in host macrophages are vacuolar compartments wherein promastigote forms differentiate into amastigote that are the replicative form of the parasite and are also more resistant to host responses. We revisited the biogenesis of tight-fitting PVs that insulate L. infantum in promastigote-infected macrophage-like RAW 264.7 cells by time-dependent confocal laser multidimensional imaging analysis. Pharmacological disassembly of the cellular microtubule network and silencing of the dynein gene led to an impaired interaction of L. infantum-containing phagosomes with late endosomes and lysosomes, resulting in the tight-fitting parasite-containing phagosomes never transforming into mature PVs. Analysis of the shape of the L. infantum parasite within PVs, showed that factors that impair promastigote-amastigote differentiation can also result in PVs whose maturation is arrested. These findings highlight the importance of the MT-dependent interaction of L. infantum-containing phagosomes with the host macrophage endolysosomal pathway to secure the intracellular fate of the parasite.
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https://www.hal.inserm.fr/inserm-02966708
Contributor : Myriam Bodescot <>
Submitted on : Wednesday, October 14, 2020 - 11:58:14 AM
Last modification on : Saturday, July 3, 2021 - 12:29:07 AM

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journal.pntd.0008396.pdf
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  • HAL Id : inserm-02966708, version 1

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Sandrine Cojean, Valérie Nicolas, Vanessa Lievin-Le Moal. The macrophage microtubule network acts as a key cellular controller of the intracellular fate of Leishmania infantum. PLoS Neglected Tropical Diseases, Public Library of Science, 2020, 14 (7), pp.e0008396. ⟨inserm-02966708⟩

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