Skip to Main content Skip to Navigation
Journal articles

Ratiometric quorum sensing governs the trade-off between bacterial vertical and horizontal antibiotic resistance propagation

Abstract : Plasmid-mediated horizontal gene transfer of antibiotic resistance and virulence in pathogenic bacteria underlies a major public health issue. Understanding how, in the absence of antibiotic-mediated selection, plasmid-bearing cells avoid being outnumbered by plasmid-free cells is key to developing counterstrategies. Here, we quantified the induction of the plasmidial sex pheromone pathway of Enterococcus faecalis to show that the integration of the stimulatory (mate-sensing) and inhibitory (self-sensing) signaling modules from the pCF10 conjugative plasmid provides a precise measure of the recipient-to-donor ratio, agnostic to variations in population size. Such ratiometric control of conjugation favors vertical plasmid transfer under low mating likelihood and allows activation of conjugation functions only under high mating likelihood. We further show that this strategy constitutes a cost-effective investment into mating effort because overstimulation produces unproductive self-aggregation and growth rate reduction. A mathematical model suggests that ratiometric control of conjugation increases plasmid fitness and predicts a robust long-term, stable coexistence of donors and recipients. Our results demonstrate how population-level parameters can control transfer of antibiotic resistance in bacteria, opening the door for biotic control strategies.
Complete list of metadata

Cited literature [30 references]  Display  Hide  Download
Contributor : Myriam Bodescot Connect in order to contact the contributor
Submitted on : Tuesday, October 13, 2020 - 11:33:37 AM
Last modification on : Friday, August 5, 2022 - 12:02:33 PM
Long-term archiving on: : Thursday, January 14, 2021 - 6:32:37 PM


Publication funded by an institution


Distributed under a Creative Commons Attribution 4.0 International License




Alvaro Banderas, Arthur Carcano, Elisa Sia, Shuang Li, Ariel B Lindner. Ratiometric quorum sensing governs the trade-off between bacterial vertical and horizontal antibiotic resistance propagation. PLoS Biology, Public Library of Science, 2020, 18 (8), pp.e3000814. ⟨10.1371/journal.pbio.3000814⟩. ⟨inserm-02965404⟩



Record views


Files downloads