Rational programming of history-dependent logic in cellular populations - Archive ouverte HAL Access content directly
Journal Articles Nature Communications Year : 2020

Rational programming of history-dependent logic in cellular populations

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Abstract

Genetic programs operating in a history-dependent fashion are ubiquitous in nature and govern sophisticated processes such as development and differentiation. The ability to systematically and predictably encode such programs would advance the engineering of synthetic organisms and ecosystems with rich signal processing abilities. Here we implement robust, scalable history-dependent programs by distributing the computational labor across a cellular population. Our design is based on standardized recombinase-driven DNA scaffolds expressing different genes according to the order of occurrence of inputs. These multicellular computing systems are highly modular, do not require cell-cell communication channels, and any program can be built by differential composition of strains containing well-characterized logic scaffolds. We developed automated workflows that researchers can use to streamline program design and optimization. We anticipate that the history-dependent programs presented here will support many applications using cellular populations for material engineering, biomanufacturing and healthcare.
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Dates and versions

inserm-02952457 , version 1 (29-09-2020)

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Ana Zúñiga, Sarah Guiziou, Pauline Mayonove, Zachary Ben Meriem, Miguel Camacho, et al.. Rational programming of history-dependent logic in cellular populations. Nature Communications, 2020, 11 (1), pp.4758. ⟨10.1038/s41467-020-18455-z⟩. ⟨inserm-02952457⟩
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