, The number of Galectin3 puncta within each cell was scored using the "Find Maxima" tool
, To assess lysosomal membrane permeabilization (LMP), co-localization study of Galectin3 puncta and LAMP1 was carried out by immunofluorescence analysis. Cells were grown on glass coverslips, fixed in ice-cold methanol at ?20 ? C for 15 min and then blocked/permeabilized with 3% BSA containing
, After washing, cells were incubated with the secondary antibody (anti-rabbit Alexa Fluor 488 antibody) for 1 h at room temperature in a dark chamber and stained with 2 µg/mL of Hoechst 33258 (H3569, Triton X-100 for 1 h. Cells were then incubated with the primary antibody overnight at 4 ? C
, For some experiments, cells were stained with 150 nM tetramethylrhodamine methyl ester (TMRM, from Molecular Probes-Life Technologies) and 5 µg/mL 4 ,6-diaminidino-2-phenylindole (DAPI, from Molecular Probes-Life Technologies) for 30 min at 37 ? C. Cytofluorometric determinations of early and late apoptotis were carried out on a MACSQuant cytometer, Cell Death Cell death was determined by measuring the plasma membrane permeability using propidium iodide dye (P4864, Sigma). Briefly, supernatant and attached cells were collected, vol.93482, 1862495.
, Cell viability Assay MTT assay was used to monitor cell viability in cells subjected to different treatments, Briefly, cells
To determine the IC50 of 20A derivative compounds, a non-linear regression curve was fit using GraphPad Prism software, Molecular Devices ,
A-V is supported by the Mexican National Council of Science and Technology (CONACYT, funding #757821), Ligue Régionale d'Aquitaine, (to M.D-M). K ,
, Agence National de la Recherche (ANR) -Projets blancs
, Flash-covid-19" LYSOMOTROP; the ERA-Net for Research on Rare Diseases
Association pour la recherche sur le cancer (ARC) ,
, Association, Le Cancer du Sein, Parlons-en!
,
, Fondation pour la Recherche Médicale (FRM); a donation by Elior
, European Research Area Network on Cardiovascular Diseases (ERA-CVD
, Gustave Roussy Odyssea, the European Union Horizon 2020 Project Oncobiome; Fondation Carrefour; High-end Foreign Expert Program in China (GDW20171100085)
, Institut National du Cancer
, HTE
, LeDucq Foundation; the LabEx Immuno-Oncology (ANR-18-IDEX-0001); the RHU Torino Lumière; the Seerave Foundation; the SIRIC Stratified Oncology Cell DNA Repair and Tumor Immune Elimination (SOCRATE); and the SIRIC Cancer Research and Personalized Medicine (CARPEM)
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