Regulated expression and function of the GABA B receptor in human pancreatic beta cell line and islets - Archive ouverte HAL Access content directly
Journal Articles Scientific Reports Year : 2020

Regulated expression and function of the GABA B receptor in human pancreatic beta cell line and islets

(1) , (1) , (1) , (2) , (1) , (3) , (1) , (1) , (1)
1
2
3
Latif Rachdi
  • Function : Correspondent author
  • PersonId : 1077722

Connectez-vous pour contacter l'auteur
Alicia Maugein
  • Function : Author
Juliette Hamroune
Stefano Marullo
Olivier Albagli
  • Function : Author
Raphael Scharfmann

Abstract

G protein-coupled receptors are seven transmembrane signaling molecules that are involved in a wide variety of physiological processes. They constitute a large protein family of receptors with almost 300 members detected in human pancreatic islet preparations. However, the functional role of these receptors in pancreatic islets is unknown in most cases. We generated a new stable human beta cell line from neonatal pancreas. This cell line, named ECN90 expresses both subunits (GABBR1 and GABBR2) of the metabotropic GABAB receptor compared to human islet. In ECN90 cells, baclofen, a specific GABAB receptor agonist, inhibits cAMP signaling causing decreased expression of beta cell-specific genes such as MAFA and PCSK1, and reduced insulin secretion. We next demonstrated that in primary human islets, GABBR2 mRNA expression is strongly induced under cAMP signaling, while GABBR1 mRNA is constitutively expressed. We also found that induction and activation of the GABAB receptor in human islets modulates insulin secretion.
Fichier principal
Vignette du fichier
s41598-020-69758-6.pdf (1.6 Mo) Télécharger le fichier
Origin : Publication funded by an institution
Loading...

Dates and versions

inserm-02945829 , version 1 (22-09-2020)

Identifiers

Cite

Latif Rachdi, Alicia Maugein, Severine Pechberty, Mathieu Armanet, Juliette Hamroune, et al.. Regulated expression and function of the GABA B receptor in human pancreatic beta cell line and islets. Scientific Reports, 2020, 10 (1), pp.13469. ⟨10.1038/s41598-020-69758-6⟩. ⟨inserm-02945829⟩
35 View
78 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More