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Optimized Sample Preparation Workflow for Improved Identification of Ghost Proteins

Abstract : Large scale proteomic strategies rely on database interrogation. Thus, only referenced proteins can be identified. Recently, Alternative Proteins (AltProts) translated from nonannotated Alternative Open reading frame (AltORFs) were discovered using customized databases. Because of their small size which confers them peptide-like physicochemical properties, they are more difficult to detect using standard proteomics strategies. In this study, we tested different preparation workflows for improving the identification of AltProts in NCH82 human glioma cell line. The highest number of identified AltProts was achieved with RIPA buffer or boiling water extraction followed by acetic acid precipitation.
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https://www.hal.inserm.fr/inserm-02941813
Contributor : Michel Salzet <>
Submitted on : Thursday, September 17, 2020 - 1:26:58 PM
Last modification on : Wednesday, March 17, 2021 - 2:32:03 PM

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Tristan Cardon, Flore Hervé, Vivian Delcourt, Xavier Roucou, Michel Salzet, et al.. Optimized Sample Preparation Workflow for Improved Identification of Ghost Proteins. Analytical Chemistry, American Chemical Society, 2019, 92 (1), pp.1122-1129. ⟨10.1021/acs.analchem.9b04188⟩. ⟨inserm-02941813⟩

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