Skip to Main content Skip to Navigation
Journal articles

Spatially-Resolved Top-down Proteomics Bridged to MALDI MS Imaging Reveals the Molecular Physiome of Brain Regions

Abstract : Tissue spatially-resolved proteomics was performed on 3 brain regions, leading to the characterization of 123 reference proteins. Moreover, 8 alternative proteins from alternative open reading frames (AltORF) were identified. Some proteins display specific post-translational modification profiles or truncation linked to the brain regions and their functions. Systems biology analysis performed on the proteome identified in each region allowed to associate sub-networks with the functional physiology of each brain region. Back correlation of the proteins identified by spatially-resolved proteomics at a given tissue localization with the MALDI MS imaging data, was then performed. As an example, mapping of the distribution of the matrix metallopeptidase 3-cleaved C-terminal fragment of α-synuclein (aa 95-140) identified its specific distribution along the hippocampal dentate gyrus. Taken together, we established the molecular physiome of 3 rat brain regions through reference and hidden proteome characterization.
Document type :
Journal articles
Complete list of metadata

Cited literature [69 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-02941492
Contributor : Michel Salzet <>
Submitted on : Thursday, September 17, 2020 - 9:55:49 AM
Last modification on : Wednesday, March 17, 2021 - 2:32:03 PM
Long-term archiving on: : Thursday, December 3, 2020 - 9:07:22 AM

File

357.full.pdf
Publication funded by an institution

Identifiers

Collections

Citation

Vivian Delcourt, Julien Franck, Jusal Quanico, Jean-Pascal Gimeno, Maxence Wisztorski, et al.. Spatially-Resolved Top-down Proteomics Bridged to MALDI MS Imaging Reveals the Molecular Physiome of Brain Regions. Molecular and Cellular Proteomics, American Society for Biochemistry and Molecular Biology, 2018, 17 (2), pp.357-372. ⟨10.1074/mcp.M116.065755⟩. ⟨inserm-02941492⟩

Share

Metrics

Record views

23

Files downloads

64