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Remote Atmospheric Pressure Infrared Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry (Remote IR-MALDI MS) of Proteins

Abstract : Remote Infrared Matrix-Assisted Laser Desorption/Ionization (Remote IR-MALDI) system using tissue endogenous water as matrix was shown to enable in vivo real-time mass spectrometry analysis with minimal invasiveness. Initially the system was used to detect metabolites and lipids. Here, we demonstrate its capability to detect and analyze peptides and proteins. Very interestingly, the corresponding mass spectra show ESI-like charge state distribution, opening many applications for structural elucidation to be performed in real-time by Top-Down strategy. The charge states show no dependence toward laser wavelength or length of the transfer tube. Indeed, remote analysis can be performed 5 m away from the mass spectrometer without modification of spectra. On the contrary, addition of glycerol to water shift the charge state distributions toward even higher charge states. The desorption/ionization process is very soft, allowing to maintain protein conformation as in ESI. Observation of proteins and similar spectral features on tissue, when protein standards are deposited on raw tissue pieces, could potentially open the way to their direct analysis from biological samples. This also brings interesting features that could contribute to the understanding of IR MALDI ionization mechanism.
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https://www.hal.inserm.fr/inserm-02940859
Contributor : Michel Salzet <>
Submitted on : Wednesday, September 16, 2020 - 3:53:33 PM
Last modification on : Friday, June 4, 2021 - 10:12:07 AM

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Benoit Fatou, Michael Ziskind, Philippe Saudemont, Jusal Quanico, Cristian Focsa, et al.. Remote Atmospheric Pressure Infrared Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry (Remote IR-MALDI MS) of Proteins. Molecular and Cellular Proteomics, American Society for Biochemistry and Molecular Biology, 2018, 17 (8), pp.1637-1649. ⟨10.1074/mcp.TIR117.000582⟩. ⟨inserm-02940859⟩

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