Skip to Main content Skip to Navigation
Journal articles

Lipid Changes Associated with Traumatic Brain Injury Revealed by 3D MALDI-MSI

Abstract : Traumatic brain injury (TBI) is a major cause of death and disability in children and young adults worldwide according to the World Health Organization (WHO). The emergence of mass-spectrometry-based techniques, such as MALDI-MSI, has allowed the monitoring and visualization of changes post injury, providing a global picture of the impact of TBI on different classes of molecules in a single study. In this work, we show the ability to track lipid changes post-TBI by three-dimensional matrix-assisted laser desorption/ionization-mass-spectrometry imaging (MALDI-MSI). Controlled cortical impact (CCI) was induced on adult male rats resulting in direct mechanical injury to the cortical tissue on the right ipsilateral hemisphere of the brain. Images of lipid distribution in coronally sectioned injured brains were acquired using a high-resolution mass spectrometer (MALDI-LTQ-Orbitrap-XL). Results reveal unique lipid signatures for the injured cortical tissue, which further segregate into two subgroups of injury (lesion interior and lesion exterior). Although both subgroups show different profiles from that of the noninjured cortical tissue, the lesion interior is more similar to the ventricular system than the lesion exterior. For example, m/ z 725.56 showed expression in both injured tissue and the ventricular system, whereas m/ z 856.59 (phosphatidylcholine 42:9) is uniquely expressed in injured tissue. On the other hand, m/ z 797.59 (also a phosphatidylcholine) showed unique expression to the ventricular system and not to the injured cortical tissue. Our results can help in further monitoring and identifying lesion-specific lipids in a 3D manner to obtain a better understanding and visualization of molecular and cellular events occurring post-TBI.
Document type :
Journal articles
Complete list of metadata

Cited literature [40 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-02940820
Contributor : Michel Salzet <>
Submitted on : Wednesday, September 16, 2020 - 3:35:33 PM
Last modification on : Wednesday, March 17, 2021 - 2:32:03 PM
Long-term archiving on: : Thursday, December 3, 2020 - 8:30:20 AM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

Collections

Citation

Khalil Mallah, Jusal Quanico, Dennis Trede, Firas Kobeissy, Kazem Zibara, et al.. Lipid Changes Associated with Traumatic Brain Injury Revealed by 3D MALDI-MSI. Analytical Chemistry, American Chemical Society, 2018, 90 (17), pp.10568-10576. ⟨10.1021/acs.analchem.8b02682⟩. ⟨inserm-02940820⟩

Share

Metrics

Record views

29