Skip to Main content Skip to Navigation
Journal articles

3D MALDI mass spectrometry imaging reveals specific localization of long-chain acylcarnitines within a 10-day time window of spinal cord injury

Abstract : We report, for the first time, the detection and specific localization of long-chain acylcarnitines (LC ACs) along the lesion margins in an experimental model of spinal cord injury (SCI) using 3D mass spectrometry imaging (MSI). Acylcarnitines palmitoylcarnitine (AC(16:0)), palmitoleoylcarnitine (AC(16:1)), elaidic carnitine (AC(18:1)) and tetradecanoylcarnitine (AC(14:1)) were detected as early as 3 days post injury, and were present along the lesion margins 7 and 10 days after SCI induced by balloon compression technique in the rat. 3D MSI revealed the heterogeneous distribution of these lipids across the injured spinal cord, appearing well-defined at the lesion margins rostral to the lesion center, and becoming widespread and less confined to the margins at the region located caudally. The assigned acylcarnitines co-localize with resident microglia/macrophages detected along the lesion margins by immunofluorescence. Given the reported pro-inflammatory role of these acylcarnitines, their specific spatial localization along the lesion margin could hint at their potential pathophysiological roles in the progression of SCI.
Document type :
Journal articles
Complete list of metadatas

Cited literature [36 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-02940797
Contributor : Michel Salzet <>
Submitted on : Wednesday, September 16, 2020 - 3:23:57 PM
Last modification on : Thursday, September 17, 2020 - 3:14:18 AM

File

s41598-018-34518-0.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Jusal Quanico, Lena Hauberg-Lotte, Stephanie Devaux, Zahra Laouby, Celine Meriaux, et al.. 3D MALDI mass spectrometry imaging reveals specific localization of long-chain acylcarnitines within a 10-day time window of spinal cord injury. Scientific Reports, Nature Publishing Group, 2018, 8 (1), pp.16083. ⟨10.1038/s41598-018-34518-0⟩. ⟨inserm-02940797⟩

Share

Metrics

Record views

35

Files downloads

37