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Journal articles
3D MALDI mass spectrometry imaging reveals specific localization of long-chain acylcarnitines within a 10-day time window of spinal cord injury
Abstract : We report, for the first time, the detection and specific localization of long-chain acylcarnitines (LC ACs) along the lesion margins in an experimental model of spinal cord injury (SCI) using 3D mass spectrometry imaging (MSI). Acylcarnitines palmitoylcarnitine (AC(16:0)), palmitoleoylcarnitine (AC(16:1)), elaidic carnitine (AC(18:1)) and tetradecanoylcarnitine (AC(14:1)) were detected as early as 3 days post injury, and were present along the lesion margins 7 and 10 days after SCI induced by balloon compression technique in the rat. 3D MSI revealed the heterogeneous distribution of these lipids across the injured spinal cord, appearing well-defined at the lesion margins rostral to the lesion center, and becoming widespread and less confined to the margins at the region located caudally. The assigned acylcarnitines co-localize with resident microglia/macrophages detected along the lesion margins by immunofluorescence. Given the reported pro-inflammatory role of these acylcarnitines, their specific spatial localization along the lesion margin could hint at their potential pathophysiological roles in the progression of SCI.
Cited literature [36 references]
https://www.hal.inserm.fr/inserm-02940797
Contributor : Michel Salzet <>
Submitted on : Wednesday, September 16, 2020 - 3:23:57 PM
Last modification on : Tuesday, November 3, 2020 - 2:24:02 PM
Long-term archiving on: : Thursday, December 3, 2020 - 8:28:46 AM
Contributor : Michel Salzet <>
Submitted on : Wednesday, September 16, 2020 - 3:23:57 PM
Last modification on : Tuesday, November 3, 2020 - 2:24:02 PM
Long-term archiving on: : Thursday, December 3, 2020 - 8:28:46 AM
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s41598-018-34518-0.pdf
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