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Real-Time Molecular Diagnosis of Tumors Using Water-Assisted Laser Desorption/Ionization Mass Spectrometry Technology

Abstract : Histopathological diagnosis of biopsy samples and margin assessment of surgical specimens are challenging aspects in sarcoma. Using dog patient tissues, we assessed the performance of a recently developed technology for fast ex vivo molecular lipid-based diagnosis of sarcomas. The instrument is based on mass spectrometry (MS) molecular analysis through a laser microprobe operating under ambient conditions using excitation of endogenous water molecules. Classification models based on cancer/normal/necrotic, tumor grade, and subtypes showed a minimum of 97.63% correct classification. Specific markers of normal, cancer, and necrotic regions were identified by tandem MS and validated by MS imaging. Real-time detection capabilities were demonstrated by ex vivo analysis with direct interrogation of classification models.
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https://www.hal.inserm.fr/inserm-02940768
Contributor : Michel Salzet <>
Submitted on : Wednesday, September 16, 2020 - 3:11:43 PM
Last modification on : Friday, June 4, 2021 - 10:12:07 AM
Long-term archiving on: : Thursday, December 3, 2020 - 8:26:23 AM

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Philippe Saudemont, Jusal Quanico, Yves-Marie Robin, Anna Baud, Julia Balog, et al.. Real-Time Molecular Diagnosis of Tumors Using Water-Assisted Laser Desorption/Ionization Mass Spectrometry Technology. Cancer Cell, Elsevier, 2018, 34 (5), pp.840-851.e4. ⟨10.1016/j.ccell.2018.09.009⟩. ⟨inserm-02940768⟩

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