Deep transcriptome annotation suggests that small and large proteins encoded in the same genes often cooperate

Sondos Samandi; Annie Roy; Vivian Delcourt; Jean-François Lucier; Jules Gagnon; Maxime Beaudoin; Benoît Vanderperre; Marc-André Breton; Julie Motard; Jean-François Jacques; Mylène Brunelle; Isabelle Gagnon-Arsenault; Isabelle Fournier; Aïda Ouangraoua; Darel Hunting; Alan Cohen; Christian Landry; Michelle Scott; Xavier Roucou

doi:10.1101/142992

Preprints, Working Papers, ...

Deep transcriptome annotation suggests that small and large proteins encoded in the same genes often cooperate

Sondos Samandi ^{1, 2, 3} Annie Roy ^{1, 2, 3} Vivian Delcourt ^{4, 1, 2, 3} Jean-François Lucier ¹ Jules Gagnon ¹ Maxime Beaudoin ^{1, 2, 3} Benoît Vanderperre ¹ Marc-André Breton ¹ Julie Motard ^{1, 2, 3} Jean-François Jacques ^{1, 2, 3} Mylène Brunelle ^{1, 2, 3} Isabelle Gagnon-Arsenault ^{2, 3} Isabelle Fournier ⁴ Aïda Ouangraoua ¹ Darel Hunting ¹ Alan Cohen ¹ Christian Landry ^{2, 3} Michelle Scott ¹ Xavier Roucou ^{1, 3}

1 Université de Sherbrooke [Sherbrooke]

2 ULaval - Université Laval [Québec]

3 PROTEO, The Quebec Network for Research on Protein Function, Engineering, and Applications

4 PRISM - Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192

Résumé : Recent studies in eukaryotes have demonstrated the translation of alternative open reading frames (altORFs) in addition to annotated protein coding sequences (CDSs). We show that a large number of small proteins could in fact be coded by altORFs. The putative alternative proteins translated from altORFs have orthologs in many species and evolutionary patterns indicate that altORFs are particularly constrained in CDSs that evolve slowly. Thousands of predicted alternative proteins are detected in proteomic datasets by reanalysis using a database containing predicted alternative proteins. Protein domains and co-conservation analyses suggest a potential functional relationship between small and large proteins encoded in the same genes. This is illustrated with specific examples, including altMiD51, a 70 amino acid mitochondrial fission-promoting protein encoded in MiD51 / Mief1 / SMCR7L , a gene encoding an annotated protein promoting mitochondrial fission. Our results suggest that many coding genes code for more than one protein that are often functionally related.

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Preprints, Working Papers, ...

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Life Sciences [q-bio]

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https://www.hal.inserm.fr/inserm-02940621
Contributor : Michel Salzet <>
Submitted on : Wednesday, September 16, 2020 - 2:08:49 PM
Last modification on : Tuesday, November 3, 2020 - 2:24:02 PM

Deep transcriptome annotation suggests that small and large proteins encoded in the same genes often cooperate

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Deep transcriptome annotation suggests that small and large proteins encoded in the same genes often cooperate

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