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Molecular and expression analysis of the Allograft inflammatory factor 1 (AIF-1) in the coelomocytes of the common sea urchin Paracentrotus lividus

Abstract : Allograft inflammatory factor 1 (AIF-1) is a highly conserved gene involved in inflammation, cloned and characterized in several evolutionary distant animal species. Here, we report the molecular identification, characterization and expression of AIF-1 from the common sea urchin Paracentrotus lividus. In this species, AIF-1 encodes a predicted 151 amino acid protein with high similarity to vertebrate AIF-1 proteins. Immunocytochemical analyses on coelomocytes reveal localization of the AIF-1 protein in amoebocytes (perinuclear cytoplasmic zone) and red sphaerulocytes (inside granules), but not in vibratile cells and colorless sphaerula cells. The significant increase of AIF-1 expression (mRNA and protein) found in the coelomocytes of the sea urchin after Gram + bacterial challenge suggests the involvement of AIF-1 in the inflammatory response. Our analysis on P. lividus AIF-1 contributes to elucidate AIF-1 function along the evolutionary scale and consolidate the key evolutionary position of echinoderms throughout metazoans with respect to the common immune paths.
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https://www.hal.inserm.fr/inserm-02940581
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Submitted on : Wednesday, September 16, 2020 - 1:40:55 PM
Last modification on : Tuesday, November 3, 2020 - 2:24:01 PM

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Amilcare Barca, Francesca Vacca, Jacopo Vizioli, Francesco Drago, Carla Vetrugno, et al.. Molecular and expression analysis of the Allograft inflammatory factor 1 (AIF-1) in the coelomocytes of the common sea urchin Paracentrotus lividus. Fish and Shellfish Immunology, Elsevier, 2017, 71, pp.136-143. ⟨10.1016/j.fsi.2017.09.078⟩. ⟨inserm-02940581⟩

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