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Standardization procedure for flow cytometry data harmonization in prospective multicenter studies

Lucas Le Lann 1 Pierre-Emmanuel Jouve 2 Marta Alarcón-Riquelme 3 Christophe Jamin 1, 4, * Jacques-Olivier Pers 1
* Corresponding author
1 LBAI - Lymphocyte B et Auto-immunité
2 AltraBio [Lyon]
3 University of Granada [Granada]
4 Laboratoire d'Immunologie et Immunothérapie [Brest]
Abstract : One of the most challenging objective for clinical cytometry in prospective multicenter immunomonitoring trials is to compare frequencies, absolute numbers of leukocyte populations and further the mean fluorescence intensities of cell markers, especially when the data are generated from different instruments. Here, we describe an innovative standardization workflow to compare all data to carry out any large-scale, prospective multicentric flow cytometry analysis whatever the duration, the number or type of instruments required for the realization of such projects.
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https://www.hal.inserm.fr/inserm-02926167
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Submitted on : Monday, August 31, 2020 - 2:31:10 PM
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Lucas Le Lann, Pierre-Emmanuel Jouve, Marta Alarcón-Riquelme, Christophe Jamin, Jacques-Olivier Pers. Standardization procedure for flow cytometry data harmonization in prospective multicenter studies. Scientific Reports, Nature Publishing Group, 2020, 10 (1), pp.11567. ⟨10.1038/s41598-020-68468-3⟩. ⟨inserm-02926167⟩

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