 |
Journal articles
AutoNeuriteJ: An ImageJ plugin for measurement and classification of neuritic extensions
Abstract : Morphometry characterization is an important procedure in describing neuronal cultures and identifying phenotypic differences. This task usually requires labor-intensive measurements and the classification of numerous neurites from large numbers of neurons in culture. To automate these measurements, we wrote AutoNeuriteJ, an imageJ/Fiji plugin that measures and classifies neurites from a very large number of neurons. We showed that AutoNeuriteJ is able to detect variations of neuritic growth induced by several compounds known to affect the neuronal growth. In these experiments measurement of more than 5000 mouse neurons per conditions was obtained within a few hours. Moreover, by analyzing mouse neurons deficient for the microtubule associated protein 6 (MAP6) and wild type neurons we illustrate that AutoNeuriteJ is capable to detect subtle phenotypic difference in axonal length. Overall the use of AutoNeuriteJ will provide rapid, unbiased and accurate measurement of neuron morphologies.
Cited literature [26 references]
https://www.hal.inserm.fr/inserm-02914636
Contributor : Myriam Bodescot <>
Submitted on : Wednesday, August 12, 2020 - 11:12:06 AM
Last modification on : Saturday, May 22, 2021 - 3:22:58 AM
Long-term archiving on: : Monday, November 30, 2020 - 6:41:41 PM
Contributor : Myriam Bodescot <>
Submitted on : Wednesday, August 12, 2020 - 11:12:06 AM
Last modification on : Saturday, May 22, 2021 - 3:22:58 AM
Long-term archiving on: : Monday, November 30, 2020 - 6:41:41 PM
File
journal.pone.0234529.pdf
Publication funded by an institution