Skip to Main content Skip to Navigation
Journal articles

AutoNeuriteJ: An ImageJ plugin for measurement and classification of neuritic extensions

Abstract : Morphometry characterization is an important procedure in describing neuronal cultures and identifying phenotypic differences. This task usually requires labor-intensive measurements and the classification of numerous neurites from large numbers of neurons in culture. To automate these measurements, we wrote AutoNeuriteJ, an imageJ/Fiji plugin that measures and classifies neurites from a very large number of neurons. We showed that AutoNeuriteJ is able to detect variations of neuritic growth induced by several compounds known to affect the neuronal growth. In these experiments measurement of more than 5000 mouse neurons per conditions was obtained within a few hours. Moreover, by analyzing mouse neurons deficient for the microtubule associated protein 6 (MAP6) and wild type neurons we illustrate that AutoNeuriteJ is capable to detect subtle phenotypic difference in axonal length. Overall the use of AutoNeuriteJ will provide rapid, unbiased and accurate measurement of neuron morphologies.
Complete list of metadata

Cited literature [26 references]  Display  Hide  Download
Contributor : Myriam Bodescot Connect in order to contact the contributor
Submitted on : Wednesday, August 12, 2020 - 11:12:06 AM
Last modification on : Wednesday, November 3, 2021 - 8:11:55 AM
Long-term archiving on: : Monday, November 30, 2020 - 6:41:41 PM


Publication funded by an institution





Benoit Boulan, Anne Beghin, Charlotte Ravanello, Jean-Christophe Deloulme, Sylvie Gory-Fauré, et al.. AutoNeuriteJ: An ImageJ plugin for measurement and classification of neuritic extensions. PLoS ONE, Public Library of Science, 2020, 15 (7), pp.e0234529. ⟨10.1371/journal.pone.0234529⟩. ⟨inserm-02914636⟩



Record views


Files downloads