Skip to Main content Skip to Navigation
Journal articles

High oncolytic activity of a double deleted Vaccinia Virus Copenhagen strain against malignant pleural mesothelioma: Mesothelioma and oncolytic vaccinia virus

Abstract : Malignant pleural mesothelioma (MPM) is a cancer of the pleura that lacks efficient treatment. Oncolytic immunotherapy using oncolytic vaccinia virus (VV) may represent an alternative therapeutic approach for the treatment of this malignancy. Here we studied the oncolytic activity of VVTK-RR-/GFP against MPM. This virus is a VV from the Copenhagen strain that is deleted of two genes encoding the thymidine kinase (J2R) and the ribonucleotide reductase (I4L) and that express the green fluorescent protein (GFP). First we show in vitro that VVTK-RR-/GFP efficiently infects and kills the twenty two human MPM cell lines used in this study. We also show that the virus replicates in all eight tested MPM cell lines, however with approximately a 10-fold differences in the amplification level from one cell line to another. Then we studied the therapeutic efficiency of VVTK-RR-/GFP in NOD Scid mice that bear peritoneal human MPM tumors. One intraperitoneal infection of VVTK-RR-/GFP reduces the tumor burden and significantly increases mice survival compared to untreated animals. Thus VVTK-RR- may be a promising OV for the oncolytic immunotherapy of MPM.
Document type :
Journal articles
Complete list of metadatas

Cited literature [22 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-02913764
Contributor : Elizabeth Bernardo <>
Submitted on : Monday, August 10, 2020 - 3:01:24 PM
Last modification on : Saturday, August 22, 2020 - 3:27:09 AM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : 2021-02-10

Please log in to resquest access to the document

Identifiers

  • HAL Id : inserm-02913764, version 1

Citation

Tiphaine Delaunay, Joëlle Nader, Marion Grard, Isabelle Farine, Vera Hedwig, et al.. High oncolytic activity of a double deleted Vaccinia Virus Copenhagen strain against malignant pleural mesothelioma: Mesothelioma and oncolytic vaccinia virus. Molecular Therapy - Oncolytics, Elsevier, 2020, Online ahead of print. ⟨inserm-02913764⟩

Share

Metrics

Record views

22