Investigating the Product Profiles and Structural Relationships of New Levansucrases with Conventional and Non-Conventional Substrates - Archive ouverte HAL Access content directly
Journal Articles International Journal of Molecular Sciences Year : 2020

Investigating the Product Profiles and Structural Relationships of New Levansucrases with Conventional and Non-Conventional Substrates

(1) , (1) , (2, 3, 4) , (2, 3, 4)
1
2
3
4

Abstract

The synthesis of complex oligosaccharides is desired for their potential as prebiotics, and their role in the pharmaceutical and food industry. Levansucrase (LS, EC 2.4.1.10), a fructosyl-transferase, can catalyze the synthesis of these compounds. LS acquires a fructosyl residue from a donor molecule and performs a non-Lenoir transfer to an acceptor molecule, via β-(2→6)-glycosidic linkages. Genome mining was used to uncover new LS enzymes with increased transfructosylating activity and wider acceptor promiscuity, with an initial screening revealing five LS enzymes. The product profiles and activities of these enzymes were examined after their incubation with sucrose. Alternate acceptor molecules were also incubated with the enzymes to study their consumption. LSs from Gluconobacter oxydans and Novosphingobium aromaticivorans synthesized fructooligosaccharides (FOSs) with up to 13 units in length. Alignment of their amino acid sequences and substrate docking with homology models identified structural elements causing differences in their product spectra. Raffinose, over sucrose, was the preferred donor molecule for the LS from Vibrio natriegens, N. aromaticivorans, and Paraburkolderia graminis. The LSs examined were found to have wide acceptor promiscuity, utilizing monosaccharides, disaccharides, and two alcohols to a high degree.
Fichier principal
Vignette du fichier
Hill_Karbourne_Narwani_de Brevern_2020_IJMS_Investigating the Product Profiles and Structural Relationships of New Levansucrases with Conventional and Non-Conventional Substrates.pdf (5.25 Mo) Télécharger le fichier
Vignette du fichier
Hill_Karbourne_Narwani_de Brevern_2020_IJMS_Investigating the Product Profiles and Structural Relationships of New Levansucrases with Conventional and Non-Conventional Substrates_sup.pdf (1.73 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Loading...

Dates and versions

inserm-02913693 , version 1 (10-08-2020)

Identifiers

Cite

Andrea Hill, Salwa Karboune, Tarun Narwani, Alexandre de Brevern. Investigating the Product Profiles and Structural Relationships of New Levansucrases with Conventional and Non-Conventional Substrates. International Journal of Molecular Sciences, 2020, 21 (15), pp.5402. ⟨10.3390/ijms21155402⟩. ⟨inserm-02913693⟩
36 View
27 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More