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Journal articles
Epigenetic homogeneity in histone methylation underlies sperm programming for embryonic transcription
Mami Oikawa
1
Angela Simeone
1
Eva Hormanseder
1
Marta Teperek
1
Vincent Gaggioli
1
Alan O'Doherty
2
Emma Falk
3
Matthieu Sporniak
3
Clive d'Santos
1
Valar Nila Roamio Franklin
1
Kamal Kishore
1
Charles Bradshaw
1
Declan Keane
4
Thomas Fréour
5
Laurent David
3
Adrian Grzybowski
6
Alexander Ruthenburg
6
John Gurdon
1
Jérôme Jullien
1, 3, *
*
Corresponding author
Clive d'Santos 1
Author
Valar Nila Roamio Franklin 1
Author
Kamal Kishore 1
Author
Charles R Bradshaw 1
Author
1
CAM - University of Cambridge [UK] (The Old Schools, Trinity Lane, Cambridge CB2 1TN - United Kingdom)
2
UCD - University College Dublin [Dublin] (Belfield, Dublin 4 - Ireland)
3
U1064 Inserm - CRTI - Centre de Recherche en Transplantation et Immunologie (CHU Nantes - Hôtel Dieu - 30 Bd Jean Monnet 44093 Nantes Cedex 01. - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1064 (101, rue de Tolbiac, 75013 Paris - France)
- UFR MEDECINE - Université de Nantes - UFR de Médecine et des Techniques Médicales (1 rue Gaston Veil BP 53508 44035 NANTES Cedex 1 - France)
- UN - Université de Nantes (1, quai de Tourville - BP 13522 - 44035 Nantes cedex 1 - France)
4
ReproMed Ireland [Dublin, Irlande] (Ireland)
5
Service de Biologie de la Reproduction [Nantes] (France)
- CHU Nantes - Centre hospitalier universitaire de Nantes (1 Place Alexis-Ricordeau, 44000 Nantes - France)
6
UIC - University of Illinois [Chicago] (1200 West Harrison St.,
Chicago, Illinois 60607 - United States)
- University of Illinois System (108 Henry Administration Bldg. Urbana, IL 61801 - United States)
Abstract : Sperm contributes genetic and epigenetic information to the embryo to efficiently support development. However, the mechanism underlying such developmental competence remains elusive. Here, we investigated whether all sperm cells have a common epigenetic configuration that primes transcriptional program for embryonic development. Using calibrated ChIP-seq, we show that remodelling of histones during spermiogenesis results in the retention of methylated histone H3 at the same genomic location in most sperm cell. This homogeneously methylated fraction of histone H3 in the sperm genome is maintained during early embryonic replication. Such methylated histone fraction resisting post-fertilisation reprogramming marks developmental genes whose expression is perturbed upon experimental reduction of histone methylation. A similar homogeneously methylated histone H3 fraction is detected in human sperm. Altogether, we uncover a conserved mechanism of paternal epigenetic information transmission to the embryo through the homogeneous retention of methylated histone in a sperm cells population.
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Journal articles
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https://www.hal.inserm.fr/inserm-02909255
Contributor : Myriam Bodescot <>
Submitted on : Thursday, July 30, 2020 - 11:02:51 AM
Last modification on : Tuesday, August 4, 2020 - 7:14:36 AM
Long-term archiving on: : Tuesday, December 1, 2020 - 9:00:05 PM
Contributor : Myriam Bodescot <>
Submitted on : Thursday, July 30, 2020 - 11:02:51 AM
Last modification on : Tuesday, August 4, 2020 - 7:14:36 AM
Long-term archiving on: : Tuesday, December 1, 2020 - 9:00:05 PM
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s41467-020-17238-w.pdf
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Identifiers
- HAL Id : inserm-02909255, version 1
- DOI : 10.1038/s41467-020-17238-w
- PUBMED : 32661239
- PUBMEDCENTRAL : PMC7359334
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Citation
Mami Oikawa, Angela Simeone, Eva Hormanseder, Marta Teperek, Vincent Gaggioli, et al.. Epigenetic homogeneity in histone methylation underlies sperm programming for embryonic transcription. Nature Communications, Nature Publishing Group, 2020, 11 (1), pp.3491. ⟨10.1038/s41467-020-17238-w⟩. ⟨inserm-02909255⟩
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