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Durable and controlled depletion of neutrophils in mice

Abstract : Neutrophils are an essential part of the innate immune system. To study their importance, experimental studies often aim to deplete these cells, generally by injecting anti-Ly6G or anti-Gr1 antibodies. However, these approaches are only partially effective, transient or lack specificity. Here we report that neutrophils remaining after anti-Ly6G treatment are newly derived from the bone marrow, instead of depletion escapees. Mechanistically, newly generated, circulating neutrophils have lower Ly6G membrane expression, and consequently reduced targets for anti-Ly6G-mediated depletion. To overcome this limitation, we develop a double antibody-based depletion strategy that enhances neutrophil elimination by anti-Ly6G treatment. This approach achieves specific, durable and controlled reduction of neutrophils in vivo, and may be suitable for studying neutrophil function in experimental models.
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https://www.hal.inserm.fr/inserm-02879178
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Submitted on : Tuesday, June 23, 2020 - 3:40:56 PM
Last modification on : Monday, June 14, 2021 - 1:58:07 PM
Long-term archiving on: : Thursday, September 24, 2020 - 6:01:29 PM

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Gaël Boivin, Julien Faget, Pierre-Benoit Ancey, Aspasia Gkasti, Julie Mussard, et al.. Durable and controlled depletion of neutrophils in mice. Nature Communications, Nature Publishing Group, 2020, 11 (1), pp.2762. ⟨10.1038/s41467-020-16596-9⟩. ⟨inserm-02879178⟩

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