Skip to Main content Skip to Navigation
Journal articles

Durable and controlled depletion of neutrophils in mice

Abstract : Neutrophils are an essential part of the innate immune system. To study their importance, experimental studies often aim to deplete these cells, generally by injecting anti-Ly6G or anti-Gr1 antibodies. However, these approaches are only partially effective, transient or lack specificity. Here we report that neutrophils remaining after anti-Ly6G treatment are newly derived from the bone marrow, instead of depletion escapees. Mechanistically, newly generated, circulating neutrophils have lower Ly6G membrane expression, and consequently reduced targets for anti-Ly6G-mediated depletion. To overcome this limitation, we develop a double antibody-based depletion strategy that enhances neutrophil elimination by anti-Ly6G treatment. This approach achieves specific, durable and controlled reduction of neutrophils in vivo, and may be suitable for studying neutrophil function in experimental models.
Document type :
Journal articles
Complete list of metadatas

Cited literature [40 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-02879178
Contributor : Myriam Bodescot <>
Submitted on : Tuesday, June 23, 2020 - 3:40:56 PM
Last modification on : Wednesday, June 24, 2020 - 3:28:04 AM

File

s41467-020-16596-9.pdf
Publication funded by an institution

Identifiers

Collections

Citation

Gaël Boivin, Julien Faget, Pierre-Benoit Ancey, Aspasia Gkasti, Julie Mussard, et al.. Durable and controlled depletion of neutrophils in mice. Nature Communications, Nature Publishing Group, 2020, 11 (1), pp.2762. ⟨10.1038/s41467-020-16596-9⟩. ⟨inserm-02879178⟩

Share

Metrics

Record views

39

Files downloads

39