Skip to Main content Skip to Navigation
Journal articles

Regeneration of segmental defects in metatarsus of sheep with vascularized and customized 3D-printed calcium phosphate scaffolds

Abstract : Although autografts are considered to be the gold standard treatment for reconstruction of large bone defects resulting from trauma or diseases, donor site morbidity and limited availability restrict their use. Successful bone repair also depends on sufficient vascularization and to address this challenge, novel strategies focus on the development of vascularized biomaterial scaffolds. This pilot study aimed to investigate the feasibility of regenerating large bone defects in sheep using 3D-printed customized calcium phosphate scaffolds with or without surgical vascularization. Pre-operative computed tomography scans were performed to visualize the metatarsus and vasculature and to fabricate customized scaffolds and surgical guides by 3D printing. Critical-sized segmental defects created in the mid-diaphyseal region of the metatarsus were either left empty or treated with the 3D scaffold alone or in combination with an axial vascular pedicle. Bone regeneration was evaluated 1, 2 and 3 months post-implantation. After 3 months, the untreated defect remained non-bridged while the 3D scaffold guided bone regeneration. The presence of the vascular pedicle further enhanced bone formation. Histology confirmed bone growth inside the porous 3D scaffolds with or without vascular pedicle inclusion. Taken together, this pilot study demonstrated the feasibility of precised pre-surgical planning and reconstruction of large bone defects with 3D-printed personalized scaffolds.
Complete list of metadatas

Cited literature [50 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-02873399
Contributor : Myriam Bodescot <>
Submitted on : Thursday, June 18, 2020 - 11:41:48 AM
Last modification on : Friday, June 19, 2020 - 3:29:34 AM

File

s41598-020-63742-w.pdf
Publication funded by an institution

Identifiers

Collections

Citation

Luciano Vidal, Carina Kampleitner, Stéphanie Krissian, Meadhbh Brennan, Oskar Hoffmann, et al.. Regeneration of segmental defects in metatarsus of sheep with vascularized and customized 3D-printed calcium phosphate scaffolds. Scientific Reports, Nature Publishing Group, 2020, 10 (1), pp.7068. ⟨10.1038/s41598-020-63742-w⟩. ⟨inserm-02873399⟩

Share

Metrics

Record views

46

Files downloads

60