Patterning of Endothelial Cells and Mesenchymal Stem Cells by Laser-Assisted Bioprinting to Study Cell Migration - Archive ouverte HAL Access content directly
Journal Articles BioMed Research International Year : 2016

Patterning of Endothelial Cells and Mesenchymal Stem Cells by Laser-Assisted Bioprinting to Study Cell Migration

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Abstract

Tissue engineering of large organs is currently limited by the lack of potent vascularization in vitro. Tissue-engineered bone grafts can be prevascularized in vitro using endothelial cells (ECs). The microvascular network architecture could be controlled by printing ECs following a specific pattern. Using laser-assisted bioprinting, we investigated the effect of distance between printed cell islets and the influence of coprinted mesenchymal cells on migration. When printed alone, ECs spread out evenly on the collagen hydrogel, regardless of the distance between cell islets. However, when printed in coculture with mesenchymal cells by laser-assisted bioprinting, they remained in the printed area. Therefore, the presence of mesenchymal cell is mandatory in order to create a pattern that will be conserved over time. This work describes an interesting approach to study cell migration that could be reproduced to study the effect of trophic factors.
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Origin : Publication funded by an institution
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Dates and versions

inserm-02870941 , version 1 (17-06-2020)

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Jean-Michel Bourget, Olivia Kérourédan, Manuela Medina, Murielle Rémy, Noélie Brunehilde Thébaud, et al.. Patterning of Endothelial Cells and Mesenchymal Stem Cells by Laser-Assisted Bioprinting to Study Cell Migration. BioMed Research International , 2016, 2016, pp.3569843. ⟨10.1155/2016/3569843⟩. ⟨inserm-02870941⟩

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