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A Unique Triculture Model to Study Osteoblasts, Osteoclasts, and Endothelial Cells

Abstract : In this article, we first developed a new medium to culture together primary human osteoblastic, osteoclastic, and endothelial cells (ECs) chosen to represent the three major bone cell tissues. Indeed, no study has been conducted on primary human cells and on the phenotype/activity retention of these three primary human cell types. Thus, we established an original triculture model with osteoblastic, osteoclastic, and ECs, where not only both cell phenotype and cell activity were maintained but also cell culture homeostasis. These promising results will permit further investigations to create in vitro conditions to mimic the bone microenvironment and analyze cell interactions in ex vivo studies.
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https://www.hal.inserm.fr/inserm-02863405
Contributor : Olivier Chassande <>
Submitted on : Wednesday, June 10, 2020 - 11:13:56 AM
Last modification on : Thursday, June 11, 2020 - 4:50:42 AM

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Agathe Gremare, Audrey Aussel, Reine Bareille, Bruno Paiva dos Santos, Joëlle Amédée, et al.. A Unique Triculture Model to Study Osteoblasts, Osteoclasts, and Endothelial Cells. Tissue Engineering Part C: Methods, Mary Ann Liebert, 2019, 25 (7), pp.421-432. ⟨10.1089/ten.TEC.2018.0301⟩. ⟨inserm-02863405⟩

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