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, CK2/HAUSP pathway is required for acquired HO development. a H&E-stained sections (left) and immunohistochemistry (right) for CSNK2A and HAUSP in human HO tissue. Scale bar, 50 ?m. b Achilles tenotomy was performed at 3-month-old male mice. Eight hours after the injury, Achilles tendon was dissected and mRNA levels of Csnk2a1, and Hausp were measured by RT-PCR
, HO was assessed by microCT and histology 3 weeks post injury. 3D-reconstruction (c) and quantification (d) and H&E-stained sections of HO areas in the muscle (e) are displayed. The arrows indicate HO in muscle. B bone, M muscle, C cartilage, BM bone marrow. Scale bars, 1 mm (c); 100 ?m (e). d n = 7 (Csnk2b fl/fl ), 7 (Osx-Cre) or 4 (Csnk2b Osx ). f-h Achilles tenotomy was performed in 3-month-old Csnk2b fl/fl , Osx-Cre, and Csnk2b Osx male mice following with a remote burn injury on the back using a heated aluminum block. HO was assessed by microCT and histology 8 weeks post injury. 3D-reconstruction (f) and quantification (g) and H&E-stained sections of HO areas in the boxes of the microCT images (h) are displayed. None non-tenotomized leg, A Achilles tendon, BM bone marrow, B bone. Scale bars, 1 mm (f); 100 ?m (h). g n = 11 (Csnk2b fl/fl ), 7 (Osx-Cre) or 6 (Csnk2b Osx ). i-l 3-month-old wild-type male mice were daily treated with DMSO (Veh) or an inhibitor of CK2 (i-CK2, 2.5 mg/kg) or HAUSP (i-HAUSP, 2 mg/kg) via intraperitoneal (i.p.) injection one day after muscle injury and BMP2/7-matrigel injection (i, j) or burn injury and Achilles tenotomy (k, l). HO was assessed by microCT analysis 3 weeks (i, j) or 8 weeks (k, l) post injury. 3D-reconstruction (i, k), HO, tenotomized Achilles tendon. (n = 4 (Csnk2a1) or 6 (Hausp)). c-e Quadriceps muscle injury by an aluminum ball drop was performed in 3-month-old Csnk2b fl/fl , Osx-Cre, and Csnk2b Osx male mice
, Data are representative of three (a-c, e, f, h, i, k) independent experiments or are pooled from two experiments (d, g, j, l). A twotailed unpaired Student's t test for comparing two groups (b, d, g) and ordinary one-way ANOVA
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