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, Downloaded from and purification are done from 50?l of each fraction (H: homogenate; MF: mitochondrial fraction; PM: pure mitochondria; MAM: mitochondria-associated membranes; ER: endoplasmic reticulum; Cyto: cytosol). Sphingolipidic compositions of the fractions are then analyzed by UPLC-MS. The results are expressed in pmol lipids/mg proteins. For ceramide, sphingomyelin, glucosylceramide and lactosylceramide the results represent the cumulative amount of all chains lengths (Mean ± SEM), at INSERM, on, 2020.
, In this image, the ER tubule undergoes a deformation at the contact region with a mitochondrion (right panel, the bar represent 100 nm). (B) After fractionation, 10 ?g total protein of each fraction are loaded on 12% SDS-PAGE to analyze the distribution of mitochondrial (VDAC, cytochrome c, TOM20), ER (CR, RTN3) and Golgi (GM130) markers. (C) Lipid extraction, www.jlr.org Downloaded from represents 0,5?m)
, Sphingolipidic compositions of the fractions are then analyzed by UPLC-MS. The results are expressed in pmol/lipids/mg proteins. For ceramide, sphingomyelin, glucosylceramide and lactosylceramide the results represent the cumulative amount of all chains lengths (Mean ± SEM). at INSERM, 2020.