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The Set1 N-terminal domain and Swd2 interact with RNA polymerase II CTD to recruit COMPASS

Abstract : Methylation of histone H3 lysine 4 (H3K4) by Set1/COMPASS occurs co-transcriptionally, and is important for gene regulation. Set1/COMPASS associates with the RNA polymerase II C-terminal domain (CTD) to establish proper levels and distribution of H3K4 methylations. However, details of CTD association remain unclear. Here we report that the Set1 N-terminal region and the COMPASS subunit Swd2, which interact with each other, are both needed for efficient CTD binding in Saccharomyces cerevisiae. Moreover, a single point mutation in Swd2 that affects its interaction with Set1 also impairs COMPASS recruitment to chromatin and H3K4 methylation. A CTD interaction domain (CID) from the protein Nrd1 can partially substitute for the Set1 N-terminal region to restore CTD interactions and histone methylation. However, even when Set1/COMPASS is recruited via the Nrd1 CID, histone H2B ubiquitylation is still required for efficient H3K4 methylation, indicating that H2Bub acts after the initial recruitment of COMPASS to chromatin.
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https://www.hal.inserm.fr/inserm-02617416
Contributor : Myriam Bodescot <>
Submitted on : Monday, May 25, 2020 - 11:25:21 AM
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Hyun Jin Bae, Marion Dubarry, Jongcheol Jeon, Luís Soares, Catherine Dargemont, et al.. The Set1 N-terminal domain and Swd2 interact with RNA polymerase II CTD to recruit COMPASS. Nature Communications, Nature Publishing Group, 2020, 11 (1), pp.2181. ⟨10.1038/s41467-020-16082-2⟩. ⟨inserm-02617416⟩

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