, Additional controls verifying the specificity and relevance of our in-vitro acute ERS model in MPH -related to Discussion. (A) RT-qPCR analyses of selected ERS UP genes and LIVER, Appendix Figure S25
, Mean Log2 FC ERS/Control is shown for every CHX dose. Two-way ANOVA with Dunnett's multiple comparison test was used to assess statistical significance
, RT-qPCR analyses of selected ERS UP genes and LIVER-ID TFs in MPH treated for 4h with vehicle (Control) or increasing thapsigargin doses (ERS) as indicated (3 independent experiments). (C) RT-qPCR analyses of Klf9 expression monitoring changes induced by acute ERS in mouse liver (n=4) or MPH (n=5) (light grey bars) or in sepsis BIM vs Control mouse liver (n=5) or MPH (n=5) (dark grey bars). One-sample t-test with BH correction for multiple testing was used to determine if the mean Log2 FC, µg/mL CHX as the reference). (B)
Heatmap showing the average H3K27ac ChIP-seq signal for regions with increased H3K27ac in MPH subjected to an inflammatory stimulus (INF, combination of 10 ng/mL IL-1? and 10 ng/mL IL-6 for 2h). Data are means from 3 independent experiments reported in, Appendix Figure S27. Acute ERS in MPH does not trigger activation of CRMs associated with an inflammatory response -related to Discussion. (A), 2010. ,
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