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, The indicated genes-of-interest were over-expressed (Myc) and knocked-down (LacZ or NR2) throughout the wing pouch. GFP-marked clones represent non-competitive clones. d Shown are confocal images of wing discs that were immunostained with anti-cleaved DCP1. Scale bar 50 ?m. e Heterotypic supercompetitor clonal analysis on Control-RNAi or NR2-RNAi depleted backgrounds. Wild-type clones are marked by GFP, among Myc expressing cells. Scale bar 100 ?m. f Quantification of the heterotypic supercompetitor clonal assays. See Supplementary Table 1 for genotypes. g Heterotypic clonal analysis in the presence or absence AP5, an inhibitor of NR2. Scale bar 100 ?m. h Quantification of the AP5 treatment assay. Error bars represent average occupancy of the indicated clones per wing pouch, b Quantification of the heterotypic supercompetitor clonal assays. c Homotypic supercompetitor assay. Diagram shows the average occupancy of Myc clones on homotypic background

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