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In vivo human PSC-derived intestinal organoids to study stem cell maintenance

Abstract : Human intestinal organoids (HIOs), derived from pluripotent stem cells, are a new tool to gain insights in gastrointestinal development, physiology and associated diseases. Herein, we present a method for orthotopic renal transplantation of HIOs in immunocompromised mice and subsequent analysis to study intestinal epithelial proliferation. In addition, we describe how to generate enteroids from transplanted HIOs. The method highlights the specific steps to successful engraftment and provides insight into the study of human intestinal stem cells.
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Contributor : Maxime MAHE Connect in order to contact the contributor
Submitted on : Thursday, May 14, 2020 - 7:08:50 PM
Last modification on : Wednesday, April 27, 2022 - 4:10:10 AM


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  • HAL Id : inserm-02578852, version 1



Vales Simon, Poling Holly, Sundaram Nambirajan, Michael A Helmrath, Maxime Mahé. In vivo human PSC-derived intestinal organoids to study stem cell maintenance. Intestinal Stem Cells, Epub ahead of print, In press. ⟨inserm-02578852⟩



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