Skip to Main content Skip to Navigation
Journal articles

Large Variability of Trophoblast Gene Expression Within and Between Human Normal Term Placentae

Abstract : Human placenta extracts are widely used in clinical and fundamental research, particularly to study the hormonal and exchange functions of the placenta. However, very little is known about the distribution of the main hormone mRNAs in the placenta as a whole. Total placenta extracts are heterogeneous in their cellular components, as they contain material of both fetal and maternal origin, and in their structure. Results vary greatly depending upon the location of the biopsy and the number of biopsies performed. We used real-time quantitative RT-PCR to determine whether transcripts corresponding to the main hormones secreted by the human placenta (e.g. hCG, HPL and PGH) are equally distributed within and between term placentae. We also measured cytokeratin 7 transcripts, which are specifically expressed in the trophoblast, and transcripts corresponding to nuclear receptors PPAR and RXR. A comparison of the results obtained with 12 different samples from each of four normal term placentae revealed that the amounts of transcripts differ considerably within and between each placenta. This emphasizes the need to study large numbers of samples when looking for significant differences in gene expression.
Complete list of metadatas

Cited literature [25 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-02556159
Contributor : Guillaume Pidoux <>
Submitted on : Monday, April 27, 2020 - 6:49:08 PM
Last modification on : Friday, November 13, 2020 - 12:30:03 PM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

Citation

Guillaume Pidoux, Pascale Gerbaud, Ingrid Laurendeau, Jean Guibourdenche, G Bertin, et al.. Large Variability of Trophoblast Gene Expression Within and Between Human Normal Term Placentae. Placenta, Elsevier, 2004, 25 (5), pp.469-473. ⟨10.1016/j.placenta.2003.10.016⟩. ⟨inserm-02556159⟩

Share

Metrics