A Shared Regulatory Element Controls the Initiation of Tcf7 Expression During Early T Cell and Innate Lymphoid Cell Developments

Christelle Harly; Devin Kenney; Yueqiang Wang; Yi Ding; Yongge Zhao; Parirokh Awasthi; Avinash Bhandoola

doi:10.3389/fimmu.2020.00470

Journal articles

A Shared Regulatory Element Controls the Initiation of Tcf7 Expression During Early T Cell and Innate Lymphoid Cell Developments

Christelle Harly ^{1, 2, 3} Devin Kenney ² Yueqiang Wang ^{2, 4} Yi Ding ² Yongge Zhao ² Parirokh Awasthi ⁵ Avinash Bhandoola ^{2, *}

* Corresponding author

1 CRCINA-ÉQUIPE 1 - Immunobiology of Human αβ and γδ T Cells and Immunotherapeutic Applications

CRCINA - Centre de Recherche en Cancérologie et Immunologie Nantes-Angers

2 NCI-NIH - National Cancer Institute [Bethesda]

3 LabEX IGO Immunothérapie Grand Ouest

4 Typhoon Biotech / BGI-Shenzhen [Shenzhen, China]

5 FNLCR - Frederick National Laboratory for Cancer Research

Abstract : The transcription factor TCF-1 (encoded by Tcf7) plays critical roles in several lineages of hematopoietic cells. In this study, we examined the molecular basis for Tcf7 regulation in T cells, innate lymphoid cells, and migratory conventional dendritic cells that we find express Tcf7. We identified a 1 kb regulatory element crucial for the initiation of Tcf7 expression in T cells and innate lymphoid cells, but dispensable for Tcf7 expression in Tcf7-expressing dendritic cells. Within this region, we identified a Notch binding site important for the initiation of Tcf7 expression in T cells but not in innate lymphoid cells. Our work establishes that the same regulatory element is used by distinct transcriptional controllers to initiate Tcf7 expression in T cells and ILCs.

Keywords : development innate lymphoid cells Tcf7 TCF-1 enhancer T cells

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Journal articles

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Life Sciences [q-bio] / Cancer

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Submitted on : Tuesday, April 14, 2020 - 8:11:13 AM
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A Shared Regulatory Element Controls the Initiation of Tcf7 Expression During Early T Cell and Innate Lymphoid Cell Developments

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