;. Buffer, . Biosciences, . Lincoln, and . Ne, blot was incubated with antibody against RIPK3 (1:1000), RIPK1 (1:500), or MLKL (1:1000). The membrane was washed three times with PBS/Tween 0.1% and incubated with a DyLight800-conjugated goat anti-rabbit antibody at a 1:15000 dilution (ThermoFisher Scientific, Belgium) or with ECL anti-rabbit IgG Horseradish Peroxidase conjugated antibody at a 1:2000 dilution (GE Healthcare, UK). Finally, proteins were visualized with a fluorescent Western scanning, Odyssey Infrared Imaging System

. Rna-extraction and . Rt-pcr, The relative expression of the gene of interest was calculated against the ?-actin gene. For classical RT-PCR, preparation of cDNA and PCR were performed using standard procedures. The sequences of primers are detailed in the Table 1. Statistical analysis. Statistical analyses were performed using SPSS Statistics, RNA was extracted using a High Pure RNA Tissue kit

, Differences between groups were assessed using the Kruskal-Wallis, the Mann-Whitney U or Student's t test, as appropriated. Rates of mouse survival were assessed using the Kaplan-Meier method and compared using the log-rank test. Results are expressed as mean ± SEM. A p-value of <0.05 was considered as statistically significant, 2019.

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