Skip to Main content Skip to Navigation
Journal articles

Sperm SPACA6 protein is required for mammalian Sperm-Egg Adhesion/Fusion

Abstract : Three genes are known to be essential for gamete adhesion/fusion (Cd9, Izumo1 and Juno). Here, we confirmed that Spaca6 null males are infertile and showed that their sperm accumulate in the perivitelline space but are unable to fuse with oocyte. Like IZUMO1, SPACA6 which is expressed by human sperm, is remained on the equatorial segment after acrosomal reaction and is involved in human fertilization since an anti-SPACA6 antibody inhibited it. Despite the similarity of the phenotypes caused by Spaca6 and Izumo1 knockouts, these are not redundant and the essential relocation of IZUMO1 is not affected by the lack of SPACA6. We propose a model in which IZUMO1 and SPACA6 would be part of a molecular complex necessary for gamete fusion and that their concomitant presence would be required for the recruitment of another essential molecular actor, such as a fusogen, for the fusion to take place.
Complete list of metadatas

Cited literature [29 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-02538018
Contributor : Myriam Bodescot <>
Submitted on : Thursday, April 9, 2020 - 10:46:21 AM
Last modification on : Wednesday, August 19, 2020 - 11:18:01 AM

File

s41598-020-62091-y.pdf
Publication funded by an institution

Identifiers

Collections

Citation

Sandrine Barbaux, Côme Ialy-Radio, Myriam Chalbi, Elisa Dybal, Méline Homps-Legrand, et al.. Sperm SPACA6 protein is required for mammalian Sperm-Egg Adhesion/Fusion. Scientific Reports, Nature Publishing Group, 2020, 10 (1), pp.5335. ⟨10.1038/s41598-020-62091-y⟩. ⟨inserm-02538018⟩

Share

Metrics

Record views

87

Files downloads

97