All lymphocytosis were superior to 3 ,
All experiments were performed with PBMC containing as a mean 80% of MCL cells. All patients provided written informed consent, validated by the Ethics Committee from the Avicenne Hospital in accordance with the Declaration of Helsinki. Patients usually received treatment shortly after sampling which made challenging to repeat experiments on samples of the same patient. Granta-519, Jeko-1, Maver, and HEK293T cell lines were purchased from the German Collection of Microorganisms and Cell Cultures, For patients with low lymphocytosis (<10.10 9 /L) B cells were purified by negative selection using RosetteSep kit (Stemcell technologies ,
, Cell culture and reagents
, Patients' cells were cryopreserved in liquid nitrogen in the presence of 10% dimethyl sulfoxide and 20% heatinactivated fetal calf serum (FCS) at Avicenne Hospital. MCL leukemic cells (3 × 10 6 cells/mL) and Granta-519, Jeko-1, Maver or HEK293T cells (1 × 10 6 cells/mL) were cultured in RPMI 1640 medium supplemented with 10%
For BCR stimulation soluble F(ab') 2 fragment of donkey antihuman IgM antibody (10 µg/mL; Jackson ImmunoResearch, Interchim, Monluçon, France) were used for the indicated periods of time, Cells were also treated with either Bortezomib (10 nM) (Janssen-Cilag, Issy-les-Moulineaux, France), Ibrutinib (PCI-32765) (100 nM-5 µM) ,
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