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Platelet-associated CD154 in immune thrombocytopenic purpura
Anne Solanilla
1
Jean-Max Pasquet
2
Jean-François Viallard
3
Cécile Contin
4
Christophe Grosset
5
Julie Déchanet-Merville
4
Maryse Dupouy
2
Marc Landry
6
Francis Belloc
2
Paquita Nurden
7
Patrick Blanco
4
Jean-François Moreau
4
Jean-Luc Pellegrin
3
Alan Nurden
7
Jean Ripoche
1
Jean-François Viallard
Cécile Contin
Julie Déchanet-Merville
Jean-François Moreau
Anne Solanilla 1
Author
Jean-Max Pasquet 2
Author
Jean-François Viallard 3
Author
Cécile Contin 4
Author
Christophe F Grosset 5
Author
Julie Déchanet-Merville 4
Author
Maryse Dupouy 2
Author
Marc Landry 6
Author
Francis Belloc 2
Author
Paquita Nurden 7
Author
Patrick Blanco 4
Author
Jean-François Moreau 4
Author
Jean-Luc Pellegrin 3
Author
Alan T. Nurden 7
Author
Jean Ripoche 1
Author
Jean-François Viallard
Author
1
Fibrose hépatique et cancer du foie (146, rue Léo Saignat 33076 Bordeaux - France)
- Université Bordeaux Segalen - Bordeaux 2 (146 rue Léo Saignat - 33076 Bordeaux cedex - France)
- IFR66 (France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : E0362 (101, rue de Tolbiac, 75013 Paris - France)
2
Transfert de Genes a Visee Therapeutique Dans les Cellules Souches (Universite Victor Segalen 146, Rue Leo Saignat 33076 BORDEAUX CEDEX - France)
- Université Bordeaux Segalen - Bordeaux 2 (146 rue Léo Saignat - 33076 Bordeaux cedex - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : E217 (101, rue de Tolbiac, 75013 Paris - France)
3
Médecine Interne (33500 PESSAC - France)
- CHU Bordeaux [Bordeaux] (place Amélie Raba Léon 33076 BORDEAUX CEDEX - France)
- Hôpital Haut-Lévêque [CHU Bordeaux] (France)
- CHU Bordeaux [Bordeaux] (place Amélie Raba Léon 33076 BORDEAUX CEDEX - France)
4
CIRID - Composantes innées de la réponse immunitaire et différenciation (bat. 1B 2e étage 146 Rue Léo Saignat - BP 14 33076 BORDEAUX CEDEX - France)
- Université Bordeaux Segalen - Bordeaux 2 (146 rue Léo Saignat - 33076 Bordeaux cedex - France)
- CNRS - Centre National de la Recherche Scientifique : UMR5164 (France)
5
Biothérapies des maladies génétiques et cancers (146 rue Léo Saignat, Bat TP zone sud, 33076 Bordeaux Cedex - France)
- Université Bordeaux Segalen - Bordeaux 2 (146 rue Léo Saignat - 33076 Bordeaux cedex - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1035 (101, rue de Tolbiac, 75013 Paris - France)
6
Physiopathologie des Reseaux Neuronaux Medullaires (Institut Francois Magendie 1, Rue Camille Saint Saens 33077 BORDEAUX CEDEX - France)
- Université Bordeaux Segalen - Bordeaux 2 (146 rue Léo Saignat - 33076 Bordeaux cedex - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : E358 (101, rue de Tolbiac, 75013 Paris - France)
7
Service d'hématologie (Place Amélie Rabat-Léon Bordeaux - France)
- CHU Bordeaux [Bordeaux] (place Amélie Raba Léon 33076 BORDEAUX CEDEX - France)
- Hôpital Haut-Lévêque [CHU Bordeaux] (France)
- CHU Bordeaux [Bordeaux] (place Amélie Raba Léon 33076 BORDEAUX CEDEX - France)
Abstract : CD40-ligand (CD154) is expressed on activated CD4+ T lymphocytes and is essential for the T cell-dependent activation of B lymphocytes. CD154 is also expressed at the activated platelet surface. In this study, we show that platelet-associated CD154 is increased in immune thrombocytopenic purpura (ITP), a disease characterized by an autoimmune response against proteins of the platelet membrane. CD154 and its messenger RNA were also present in increased amounts in the megakaryocytes of patients with ITP. We found that platelet-associated CD154 is competent to induce the CD40-dependent proliferation of B lymphocytes, and we observed an in vitro CD154-dependent production of antibodies to the GPIIb/IIIa complex (integrin alphaIIbbeta3) when platelets and peripheral blood B lymphocytes from ITP patients with circulating anti-GPIIb/IIIa antibody were cultured together. Therefore, platelet-associated CD154 expression is increased in ITP and is able to drive the activation of autoreactive B lymphocytes in this disease.
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Journal articles
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https://www.hal.inserm.fr/inserm-02519491
Contributor : Christophe Grosset <>
Submitted on : Thursday, March 26, 2020 - 9:54:08 AM
Last modification on : Friday, March 27, 2020 - 1:58:33 AM
Contributor : Christophe Grosset <>
Submitted on : Thursday, March 26, 2020 - 9:54:08 AM
Last modification on : Friday, March 27, 2020 - 1:58:33 AM
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- HAL Id : inserm-02519491, version 1
- DOI : 10.1182/blood-2003-07-2367
- PUBMED : 15191945
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Citation
Anne Solanilla, Jean-Max Pasquet, Jean-François Viallard, Cécile Contin, Christophe Grosset, et al.. Platelet-associated CD154 in immune thrombocytopenic purpura. Blood, American Society of Hematology, 2005, 105 (1), pp.215-218. ⟨10.1182/blood-2003-07-2367⟩. ⟨inserm-02519491⟩
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