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In Vivo Studies of Translational Repression Mediated by the Granulocyte-Macrophage Colony-stimulating Factor AU-rich Element

Abstract : The AU-rich element (ARE) controls the turnover of many unstable mRNAs and their translation. The granulocyte-macrophage colony-stimulating factor (GM-CSF) ARE is known to be a destabilizing element, but its role in translation remains unclear. Here we studied in vivo the role of the GM-CSF ARE on the mRNA and protein expressions of an enhanced green fluorescent protein reporter gene. The GM-CSF ARE had a repressor effect on translation independently of its effect on mRNA levels. In the context of an internal ribosome entry site, the GM-CSF ARE still repressed translation but was no longer functional as a destabilizing element. Gel retardation assays showed that poly(A)-binding protein is displaced from the poly(A) tail when the ARE is present in the 3'-untranslated region. These data suggest that the GM-CSF ARE controls translation and mRNA decay by interfering with poly(A)-binding protein-mediated mRNA circularization.
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https://www.hal.inserm.fr/inserm-02519489
Contributor : Christophe Grosset <>
Submitted on : Thursday, March 26, 2020 - 9:52:34 AM
Last modification on : Tuesday, September 1, 2020 - 3:13:31 AM

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Christophe Grosset, Rachel Boniface, Pascale Duchez, Anne Solanilla, Bertrand Cosson, et al.. In Vivo Studies of Translational Repression Mediated by the Granulocyte-Macrophage Colony-stimulating Factor AU-rich Element. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2004, 279 (14), pp.13354-13362. ⟨10.1074/jbc.M308003200⟩. ⟨inserm-02519489⟩

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