, Eomes and PLZF expression were analyzed in innate CD8(+) T cells and iNKT cells, respectively, among peripheral blood mononuclear cells (PBMCs) by flow cytometry ex vivo after cellular permeabilization. Eomes expression and PLZF were analyzed after gating on killer cell Ig-like receptor (KIR)/ NKG2A(+) CD8(+) CD3(+) cells and 6B11(+) CD3(+) cells, respectively. Mean fluorescence intensity (MFI) values are expressed relative to that of isotype control. Data from healthy donor (n = 5), chronic myeloid leukemia (CML)-CP (n = 6), and CML-imatinib mesylate (n = 3) were pooled. The MFI of PLZF-expressing iNKT, FigUre 4 | (a) Positive correlation between invariant natural killer T (iNKT) cell promyelocytic leukemia zinc finger (PLZF) expression and innate CD8 T cell Eomes expression
, 0043). (B-D) Innate CD8(+) T cells depend on IL-4 for homeostasis/expansion. PBMCs from five HDs were cultured for 7 days with IL-4 or medium alone (not stimulated, NS) and then analyzed by flow cytometry ex vivo after cellular permeabilization for innate CD8(+) T cells. Frequency (B), absolute cell number (c), and Eomes MFI (D) (mean ± SEM) of CD3(+) CD8(+) cells among, p.0
, CD8(+) CD3(+) cells among CD3(+) CD8(+) cells (right) are shown. Statistical significance was determined by the one-tailed Wilcoxon non-parametric test
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