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. Ko-mice, and in human control astrocytes differentiated from induced pluripotent stem cells (hAstro). b, Schematic drawing illustrating the role of OPHN1 in the RhoA/ROCK/MLC2 phosphorylation pathway. OPHN1 stimulates the GTPase activity of RhoA to its GDP bound inactivated form and turns-off the signaling from RhoA to ROCK and MLC2. Empty and red filled triangles, without or with phosphate residues. c, Western blot illustrating the phosphorylation of MLC in unscratched conditions (AE), and after 20 minutes and 24 hours following wounds on WT and OPHN1 KO astrocytes. d-e, Quantification of MLC2 phosphorylation levels in unscratched (AE) and scratched cells

, KO, n=6, p=0.0303, Mann-Whitney-test and e: WT: n=5

. Ko, Astrocytes from Ophn1 conditional model (loxKO; , Quantification of OPHN1 expression (in green) and MLC2 phosphorylation levels (in blue) in untreated (t=0) and 4OH-T treated cells

. Ko, ANOVA-test

. Ko, Asterisks indicate statistical significance